SMARCC1 Antibody (1R7I6)

Name: SMARCC1 Antibody (1R7I6)
Brand: Novus Biologicals
SKU: NBP3-16367
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16367

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1R7I6 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 700-800 of human SMARCC1 (Q92922). AFLASVVDPRVASAAAKAALEEFSRVREEVPLELVEAHVKKVQEAARASGKVDPTYGLESSCIAGTGPDEPEKLEGAEEEKMEADPDGQQPEKAENKVENE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMARCC1 Antibody (1R7I6)

Western Blot: SMARCC1 Antibody (1R7I6) [NBP3-16367] -

Western Blot: SMARCC1 Antibody (1R7I6) [NBP3-16367] - Western blot analysis of lysates from wild type (WT) and SMARCC1 knockout (KO) 293T cells using [KO Validated] SMARCC1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

Western Blot: SMARCC1 Antibody (1R7I6) [NBP3-16367]

Western Blot: SMARCC1 Antibody (1R7I6) [NBP3-16367] - Western blot analysis of extracts of various cell lines, using SMARCC1 Rabbit mAb (NBP3-16367) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Chromatin Immunoprecipitation: SMARCC1 Antibody (1R7I6) [NBP3-16367] -

Chromatin Immunoprecipitation: SMARCC1 Antibody (1R7I6) [NBP3-16367] - Chromatin immunoprecipitation analysis of extracts of MCF7 beta-estradiol cells, using SMARCC1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Immunocytochemistry/ Immunofluorescence: SMARCC1 Antibody (1R7I6) [NBP3-16367] -

Immunocytochemistry/ Immunofluorescence: SMARCC1 Antibody (1R7I6) [NBP3-16367] - Immunofluorescence analysis of NIH-3T3 cells using SMARCC1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Applications for SMARCC1 Antibody (1R7I6)

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:100 - 1:500

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SMARCC1

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCC1/BAF155 is part of the BAF complex that associates with SMARCA2/Brm and SMARCA4/Brg. The mouse homolog is Srg3 and has been found to be required for embryogenesis and brain development. SMARCC1 is also identified as SWI/SNF complex 155 kDa subunit, and BRG1-associated factor 155.

Alternate Names

BAF155SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily C member 1, BRG1-associated factor 155, chromatin remodeling complex BAF155 subunit, CRACC1, mammalian chromatin remodeling complex BRG1-associated factor 155, Rsc8, SRG3, subfamily c, member 1, SWI/SNF complex 155 kDa subunit, SWI/SNF complex subunit SMARCC1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI3

Gene Symbol

SMARCC1

Additional SMARCC1 Products

Product Documents for SMARCC1 Antibody (1R7I6)

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Certificate of Analysis

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Product Specific Notices for SMARCC1 Antibody (1R7I6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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