Smith Antigen Antibody (Y12)

Novus Biologicals | Catalog # NB600-546

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Drosophila, Rabbit

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunomicroscopy, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # Y12
View all Smith Antigen Primary Antibodies »

Product Specifications

Immunogen

The immunogen is generated from human Smith Antigen.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 17473171). Canine (PMID: 10330151). Mouse reactivity reported in scientific literature (PMID: 28093526).Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Specificity

Ab-1 recognizes cross-reactive epitopes on the B'/B and D polypeptides of Sm. Ab-1 precipitates the small nuclear RNAs U1, U2, U4, U5, and U6 providing direct evidence that the Sm antigen resides on all these RNA-protein complexes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for Smith Antigen Antibody (Y12)

Western Blot: Smith Antigen Antibody (Y12) [NB600-546]

Western Blot: Smith Antigen Antibody (Y12) [NB600-546]

Western Blot: Smith Antigen Antibody (Y12) [NB600-546] - Analysis of LS174T Cells.
Immunohistochemistry-Paraffin: Smith Antigen Antibody (Y12) [NB600-546]

Immunohistochemistry-Paraffin: Smith Antigen Antibody (Y12) [NB600-546]

Immunohistochemistry-Paraffin: Smith Antigen Antibody (Y12) [NB600-546] - Human tonsil stained with Smith antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.

Applications for Smith Antigen Antibody (Y12)

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:50 - 1:100

Immunomicroscopy

1:10 - 1:500

Western Blot

0.5 - 1.0 ug/ml
Application Notes
WB: Detects an approx. 25 kDa, which corresponds to the predicted molecular weight of Smith Antigen.Precipitates the small nuclear RNAs U1, U2, U4, U5, and U6 providing direct evidence that the Sm antigen resides on all of these RNA-protein complexes. Use in ELISA reported in scientific literature (PMID 28093526). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28515487).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4) and 0.2% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Smith Antigen

The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE.

Alternate Names

Smith antigen

Additional Smith Antigen Products

Product Documents for Smith Antigen Antibody (Y12)

Certificate of Analysis

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Product Specific Notices for Smith Antigen Antibody (Y12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Smith Antigen Antibody (Y12)

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Protocols

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FAQs

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