Smoothelin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-37931

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Predicted:

Mouse (97%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Smoothelin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LAGLDEGALRKLLEVTADLAERRRIRSAIRELQRQELEREEEALASKRFRAERQDNKENWLHSQQREAEQRAALARLAGQLESMNDVEELTALLR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Smoothelin Antibody - BSA Free

Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931]

Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931]

Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931] - Staining in human prostate and liver tissues using NBP2-37931 antibody. Corresponding SMTN RNA-seq data are presented for the same tissues.
Western Blot: Smoothelin Antibody [NBP2-37931]

Western Blot: Smoothelin Antibody [NBP2-37931]

Western Blot: Smoothelin Antibody [NBP2-37931] - Analysis in human cell lines PC-3 and HeLa using anti-SMTN antibody. Corresponding SMTN RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931]

Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931]

Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931]

Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931]

Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931]

Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931]

Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931]

Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931]

Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for Smoothelin Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Simple Western

1:100

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. See Simple Western Antibody Database for Simple Western validation: Tested in Bladder, separated by Size, antibody dilution of 1:100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smoothelin

Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are a useful tool to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein. Cells with SMC like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin and vimentin. Smoothelin colocalizes with actin stress fibers. Two tissue specific isoforms have been identified: a 59 kDa isoform specific for visceral SMC; and a 100 kDa specific for vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22. The distribution of smoothelin positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions.

Alternate Names

SMSMO, SMTN

Gene Symbol

SMTN

UniProt

P53814

Additional Smoothelin Products

Product Documents for Smoothelin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Smoothelin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Smoothelin Antibody - BSA Free

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Protocols

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FAQs

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