SMPD3/NSMase2 Antibody

Novus Biologicals | Catalog # NBP3-23413

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SMPD3 Primary Antibodies »

Product Specifications

Immunogen

SMPD3/NSMase2 synthetic peptide corresponds to amino acids in the C-terminal region of human SMPD3/NSMase2. This sequence is highly conserved in rat and mouse SMPD3/NSMase2, and has low homology to other nSMase family members. [UniProt# Q9NY59]

Specificity

The antibody detects a 78 kDa band corresponding to SMPD3/NSMase2 on SDS-PAGE immunoblots of adult mouse brain and rat PC12 cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for SMPD3/NSMase2 Antibody

SMPD3/NSMase2 Antibody

Western Blot: SMPD3/NSMase2 Antibody [NBP3-23413] -

Western Blot: SMPD3/NSMase2 Antibody [NBP3-23413] - Western blot of adult mouse brain. The blots were probed with SMPD3/NSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (lane 4).

Applications for SMPD3/NSMase2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: SMPD3

NSMase2 catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward EA2 peptide substrate, but has a weak activity towar

Long Name

Sphingomyelin Phosphodiesterase 3

Alternate Names

NSMASE2

Gene Symbol

SMPD3

Additional SMPD3 Products

Product Documents for SMPD3/NSMase2 Antibody

Certificate of Analysis

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Product Specific Notices for SMPD3/NSMase2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SMPD3/NSMase2 Antibody

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Protocols

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FAQs

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