SMYD3 Antibody (HL2464) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25688

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2464 expressed in HEK293

Format

Azide and BSA Free
View all SMYD3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human SMYD3.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMYD3 Antibody (HL2464) - Azide and BSA Free

SMYD3 Antibody (HL2464) - Azide and BSA Free

Western Blot: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] -

Western Blot: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (NBP3-25688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
SMYD3 Antibody (HL2464) - Azide and BSA Free

Western Blot: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] -

Western Blot: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (NBP3-25688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
SMYD3 Antibody (HL2464) - Azide and BSA Free

Immunohistochemistry-Paraffin: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] -

Immunohistochemistry-Paraffin: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] - SMYD3 antibody [HL2464] detects SMYD3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. SMYD3 stained by SMYD3 antibody [HL2464] (NBP3-25688) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SMYD3 Antibody (HL2464) - Azide and BSA Free

Immunohistochemistry-Paraffin: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] -

Immunohistochemistry-Paraffin: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] - SMYD3 antibody [HL2464] detects SMYD3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. SMYD3 stained by SMYD3 antibody [HL2464] (NBP3-25688) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SMYD3 Antibody (HL2464) - Azide and BSA Free

Western Blot: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] -

Western Blot: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] - Whole zebrafish extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (NBP3-25688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
SMYD3 Antibody (HL2464) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: SMYD3 Antibody (HL2464) [NBP3-25688] -

SMYD3 antibody [HL2464] detects SMYD3 protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMYD3 stained by SMYD3 antibody [HL2464] (NBP3-25688) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.

Applications for SMYD3 Antibody (HL2464) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMYD3

SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.[supplied by OMIM]

Long Name

Histone-lysine N-methyltransferase SMYD3

Alternate Names

ZMYND1, ZNFN3A1

Gene Symbol

SMYD3

Additional SMYD3 Products

Product Documents for SMYD3 Antibody (HL2464) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SMYD3 Antibody (HL2464) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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