SMYD4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81356

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ACQTEAHRMAAGPRWEAFCCNSCGAPMQGDDVLRCGSRSCAESAVSRDHLVSRLQDLQQQVRVAQKLLRDGELERAVQRLSGCQR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMYD4 Antibody - BSA Free

Western Blot: SMYD4 Antibody [NBP1-81356]

Western Blot: SMYD4 Antibody [NBP1-81356]

Western Blot: SMYD4 Antibody [NBP1-81356] - Analysis in control (vector only transfected HEK293T lysate) and SMYD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: SMYD4 Antibody [NBP1-81356]

Immunocytochemistry/ Immunofluorescence: SMYD4 Antibody [NBP1-81356]

Immunocytochemistry/Immunofluorescence: SMYD4 Antibody [NBP1-81356] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, the Golgi apparatus & vesicles.
Immunohistochemistry-Paraffin: SMYD4 Antibody [NBP1-81356]

Immunohistochemistry-Paraffin: SMYD4 Antibody [NBP1-81356]

Immunohistochemistry-Paraffin: SMYD4 Antibody [NBP1-81356] - Staining of human ovary shows cytoplasmic positivity in follicle cells.

Applications for SMYD4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMYD4

Alternate Names

KIAA1936ZMYND21, SET and MYND domain containing 4, SET and MYND domain-containing protein 4

Entrez Gene IDs

114826 (Human)

Gene Symbol

SMYD4

UniProt

Additional SMYD4 Products

Product Documents for SMYD4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SMYD4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SMYD4 Antibody - BSA Free

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  • Q: I have bought two different antibodies against SMYD4 (from two different companies) and they didn't work specifically. They were unable to detect the human SMYD4 protein that I was over-expressing (neither WB nor IF). Can you send me an aliquot to test your antibody? Do you know if it recognize the mouse Smyd4?

    A:

    I can confirm that the antibody NBP1-81356 was raised against and binds to the human SMYD4. The antibody NBP1-79406 also targets human SMYD4. Please see the images on the website datasheets for these products for validation. We also sell SMYD4 Overexpression Lysate (NBL1-16263), for use as positive control. Unfortunately, I am not able to offer you samples of these products. However, if you use them in Western blot you would be covered by our Novus Guarantee, and if you use a product in an application not mentioned on its datasheet (e.g. ICC) you would be eligible for our Innovators Reward Program.

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