SNAP23 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83162

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SNAP23 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QINKDMRETEKTLTELNKCCGLCVCPCNRTKNFESGKAYKTTWGDGGENSPCNVVSKQPGPVTNGQLQQPTTGAASGGYIKRITNDAREDEMEENLTQVGSILGNLKDMALNIGNEIDAQNPQIKRITDKADTNRDRIDIANA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%). Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SNAP23 Antibody - BSA Free

Western Blot: SNAP23 Antibody [NBP1-83162]

Western Blot: SNAP23 Antibody [NBP1-83162]

Western Blot: SNAP23 Antibody [NBP1-83162] - Analysis in human cell line HepG2.
Immunocytochemistry/ Immunofluorescence: SNAP23 Antibody [NBP1-83162]

Immunocytochemistry/ Immunofluorescence: SNAP23 Antibody [NBP1-83162]

Immunocytochemistry/Immunofluorescence: SNAP23 Antibody [NBP1-83162] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
Immunohistochemistry-Paraffin: SNAP23 Antibody [NBP1-83162]

Immunohistochemistry-Paraffin: SNAP23 Antibody [NBP1-83162]

Immunohistochemistry-Paraffin: SNAP23 Antibody [NBP1-83162] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.

Applications for SNAP23 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence fixation permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SNAP23

Synaptosomal-associated proteins (SNAPs) are cytosolic proteins that play a key role in in the process of membrane fusion in intracellular vesicle trafficking. In eukaryotic cells, the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex is critical to membrane docking and fusion and is believed to impart some degree of specificity between vesicle SNARE (v-SNARE) and target organelle SNARE (t-SNARE). In neurons and neuroendocrine cells, the SNARE complex consists of the integral membrane proteins VAMP (vesicle-associated membrane protein), syntaxin and SNAP-25. In non-neuronal tissue, a SNAP-25 homolog, SNAP-23, functionally replaces SNAP-25 in the SNARE complex. Studies show that VAMP, syntaxin and SNAP-23 are required for SNARE function and that this complex exists as a heterotrimer of the three proteins. In insulin signalling pathways, studies suggest that the translocation of GLUT4 in adipocytes requires that functional SNAP-23 be present in SNARE complexes.

Long Name

Synaptosomal-associated Protein, 23kDa

Alternate Names

HsT17016, SNAP-23, SNAP23A, SNAP23B, synaptosomal-associated protein 23, synaptosomal-associated protein, 23kD, synaptosomal-associated protein, 23kDa, Vesicle-membrane fusion protein SNAP-23

Entrez Gene IDs

8773 (Human)

Gene Symbol

SNAP23

UniProt

O00161 (Human)

Additional SNAP23 Products

Product Documents for SNAP23 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SNAP23 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Markers

Citations for SNAP23 Antibody - BSA Free

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Protocols

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FAQs

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