SNAP25 Antibody (4E11) - BSA Free

Novus Biologicals | Catalog # NBP1-04344

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E11

Format

BSA Free
View all SNAP25 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human SNAP25 (1-206aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for SNAP25 Antibody (4E11) - BSA Free

Western Blot: SNAP25 Antibody (4E11)BSA Free [NBP1-04344]

Western Blot: SNAP25 Antibody (4E11)BSA Free [NBP1-04344]

Western Blot: SNAP25 Antibody (4E11) [NBP1-04344] - The extracts of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human SNAP25 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (4E11) - BSA Free [NBP1-04344]

Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (4E11) - BSA Free [NBP1-04344]

Immunocytochemistry/Immunofluorescence: SNAP25 Antibody (4E11) [NBP1-04344] - Analysis of SNAP25 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SNAP25 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: SNAP25 Antibody (4E11) - BSA Free [NBP1-04344]

Flow Cytometry: SNAP25 Antibody (4E11) - BSA Free [NBP1-04344]

Flow Cytometry: SNAP25 Antibody (4E11) [NBP1-04344] - Analysis of SNAP25 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Western Blot: SNAP25 Antibody (4E11)BSA Free [NBP1-04344]

Western Blot: SNAP25 Antibody (4E11)BSA Free [NBP1-04344]

Western Blot: SNAP25 Antibody (4E11) [NBP1-04344] - The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SNAP25 Transfected 293T cell lysate

Applications for SNAP25 Antibody (4E11) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-1:3000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SNAP25

The synaptosomal-associated protein (SNAP25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP25 is directly involved in the release of neurotransmitters.

Long Name

Synaptosomal-associated Protein, 25 kDa

Alternate Names

RIC4, SEC9, SUP

Gene Symbol

SNAP25

UniProt

NP_570824 (Human)

Additional SNAP25 Products

Product Documents for SNAP25 Antibody (4E11) - BSA Free

Certificate of Analysis

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Product Specific Notices for SNAP25 Antibody (4E11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Markers

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Protocols

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