SNAP25 Antibody (OTI4G5)

Novus Biologicals | Catalog # NBP2-00795

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4G5
View all SNAP25 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SNAP25 (NP_003072) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

23.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SNAP25 Antibody (OTI4G5)

Western Blot: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Western Blot: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Western Blot: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.
Immunocytochemistry/Immunofluorescence: SNAP25 Antibody (4G5) [NBP2-00795] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25.
Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Human liver tissue using anti-SNAP25 mouse monoclonal antibody.
Flow Cytometry: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Flow Cytometry: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Flow Cytometry: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAP25 antibody, and then analyzed by flow cytometry.
Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SNAP25 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SNAP25 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAP25 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SNAP25 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]

Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Human colon tissue using anti-SNAP25 mouse monoclonal antibody.

Applications for SNAP25 Antibody (OTI4G5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.9 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SNAP25

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq].

Long Name

Synaptosomal-associated Protein, 25 kDa

Alternate Names

RIC4, SEC9, SUP

Entrez Gene IDs

6616 (Human)

Gene Symbol

SNAP25

Additional SNAP25 Products

Product Documents for SNAP25 Antibody (OTI4G5)

Certificate of Analysis

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Product Specific Notices for SNAP25 Antibody (OTI4G5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Markers

Citations for SNAP25 Antibody (OTI4G5)

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Protocols

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