SNF5 Antibody (CL14031) - BSA Free

Novus Biologicals | Catalog # NBP3-24595

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL14031

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q12824, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SNF5 Antibody (CL14031) - BSA Free

Immunohistochemistry-Paraffin: SNF5 Antibody (CL14031) [NBP3-24595]

Staining of human lymph node shows strong nuclear positivity in non-germinal and germinal center cells.

Immunohistochemistry-Paraffin: SNF5 Antibody (CL14031) [NBP3-24595]

Staining of human ovarian cancer (papillary carcinoma) shows strong nuclear positivity in tumor cells.

Immunocytochemistry/Immunofluorescence: SNF5 Antibody (CL14031) [NBP3-24595]

Staining in RH-30 cell line with Anti-SMARCB1 monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Western Blot: SNF5 Antibody (CL14031) [NBP3-24595]

Western blot analysis in human cell line SK-MEL-30.

Immunohistochemistry-Paraffin: SNF5 Antibody (CL14031) [NBP3-24595]

Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: SNF5 Antibody (CL14031) [NBP3-24595]

Staining of human prostate shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: SNF5 Antibody (CL14031) [NBP3-24595]

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Immunohistochemistry-Paraffin: SNF5 Antibody (CL14031) [NBP3-24595]

Staining of human endometrial cancer shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: SNF5 Antibody (CL14031) [NBP3-24595]

Staining of human colon shows strong nuclear positivity in glandular cells and lymphoid cells.

Applications for SNF5 Antibody (CL14031) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000-1:2500

Immunohistochemistry-Paraffin

1:1000-1:2500

Western Blot

1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SNF5

SNF5 is encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

BAF47, BAF47hSNF5, BRG1-associated factor 47, hSNFS, Ini1, INI1RTPS1, Integrase interactor 1 protein, malignant rhabdoid tumor suppressor, RDTSNF5 homolog, Sfh1p, SNF5, SNF5L1, Snr1, subfamily b, member 1, sucrose nonfermenting, yeast, homolog-like 1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily B member 1

Gene Symbol

SMARCB1

Additional SNF5 Products

Product Documents for SNF5 Antibody (CL14031) - BSA Free

Certificate of Analysis

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Product Specific Notices for SNF5 Antibody (CL14031) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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