SOAT1 Antibody - BSA Free

Novus Biologicals | Catalog # NB400-141

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Hamster

Cited:

Human, Mouse, Hamster

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SOAT1 Primary Antibodies »

Product Specifications

Immunogen

A fusion protein construct that contains the N-terminal region of human SOAT1. [UniProt# P35610]

Localization

Endoplasmic reticulum, membrane

Specificity

This product is specific for protein corresponding to SOAT1 Uniprot# P35610, Gene ID# 6646. [Important Note:- ACAT1 is a symbol for both acyl-Coenzyme A: cholesterol acyltransferase 1 (SOAT1/ACAT1) and for acetyl-CoA acetyltransferase 1 (ACAT1/ THIL, Gene ID 38, Uniprot P24752) which often cause confusion].

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for SOAT1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature

Immunoprecipitation

2-10 ug/ml lysate

Western Blot

1:400
Application Notes
In Western blot a band is observed at ~46 kDa representing SOAT1 along with a non-specific band at ~30 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SOAT1

SOAT1 (sterol O-acyltransferase 1), also designated ACAT1, is a homotetrameric enzyme that catalyzes the formation of cholesterol esters from cholesterol and long-chain fatty acyl coenzyme A. The gene encoding human SOAT1 maps to chromosome 1 and is expressed as a protein that localizes to the endoplasmic reticulum (ER) in several tissues, including liver, kidney, adrenal glands and macrophages. SOAT1 is involved in cellular cholesterol homeostasis as well as in foam cell formation and the subsequent progression of atherosclerosis. Several SOAT inhibitors have been developed for the treatment of atherosclerosis. SOAT2 (sterol O-acyltransferase 2), also known as ACAT2 (acyl coenzyme A), participates in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. SOAT2 is an integral membrane protein that localizes to the ER of human intestinal cells. SOAT2 deficiency contributes to severe mental retardation and hypotonus.

Long Name

Sterol O-Acyltransferase 1

Alternate Names

ACACT, ACAT-1, EC 2.3.1.26, SOAT1

Entrez Gene IDs

6646 (Human); 20652 (Mouse)

Gene Symbol

SOAT1

UniProt

P35610 (Human)

Additional SOAT1 Products

Product Documents for SOAT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SOAT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

Citations for SOAT1 Antibody - BSA Free

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Protocols

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