SOAT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92471

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SOAT1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SOAT1 (NP_003092.4). MVGEEKMSLRNRLSKSRENPEEDEDQRNPAKESLETPSNGRIDIKQLIAKKIKLTAEAEELKPFFMKEVGSHFDDFVTNLIEKSASLDNGGCALTTFSVLEGEKNNHRAKDLRAPPEQGKIFIARRSLLD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOAT1 Antibody - BSA Free

Western Blot: SOAT1 AntibodyBSA Free [NBP2-92471]

Western Blot: SOAT1 AntibodyBSA Free [NBP2-92471]

Western Blot: ACAT Antibody [NBP2-92471] - Analysis of extracts of various cell lines, using ACAT at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: SOAT1 Antibody - BSA Free [NBP2-92471]

Immunocytochemistry/ Immunofluorescence: SOAT1 Antibody - BSA Free [NBP2-92471]

Immunocytochemistry/Immunofluorescence: ACAT Antibody [NBP2-92471] - Analysis of C6 cells using SOAT1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: SOAT1 Antibody - BSA Free [NBP2-92471]

Immunocytochemistry/ Immunofluorescence: SOAT1 Antibody - BSA Free [NBP2-92471]

Immunocytochemistry/Immunofluorescence: ACAT Antibody [NBP2-92471] - Analysis of U2OS cells using SOAT1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: SOAT1 Antibody - BSA Free [NBP2-92471]

Immunocytochemistry/ Immunofluorescence: SOAT1 Antibody - BSA Free [NBP2-92471]

Immunocytochemistry/Immunofluorescence: ACAT Antibody [NBP2-92471] - Analysis of L929 cells using SOAT1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for SOAT1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SOAT1

Acely-CoA:cholestorl acyltransferase (ACAT) is an integral membrane enzyme that belongs to type-B carboxylesterase/lipase family. ACAT catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty-acyl-coenzyme A (1). ACAT also function as a regulator of intracellular cholesterol homeostasis at single cell level. Two isoform of ACAT exists, ACAT1 and ACAT2, are expressed in liver and intestine. However, ACAT1 is only found in macrophages and SMCs (2). The accumulation of cholesteryl esters produced by ACAT in macrophages can lead to foam cell formation, an indication of early stage atherosclerosis. ACAT activity has been linked to Alzehimer's Diease, condition caused by excess amount of amyloid beta-peptide (Abeta). Atherogenic lipoproteins activate ACAT activity, where active ACAT can modulate the level of Abeta (3).

Long Name

Sterol O-Acyltransferase 1

Alternate Names

ACACT, ACAT-1, EC 2.3.1.26, SOAT1

Gene Symbol

SOAT1

Additional SOAT1 Products

Product Documents for SOAT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SOAT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

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Protocols

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