SOCS-4 Antibody

Novus Biologicals | Catalog # NB100-898

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SOCS-4 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SKVRVLRIDAPEQQ corresponding to C-Terminus according to NP_543143.1, NP_955453.1.

Specificity

Both variants represent identical protein (NP_543143.1 and NP_955453.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SOCS-4 Antibody

Immunohistochemistry-Paraffin: SOCS-4 Antibody [NB100-898]

Immunohistochemistry-Paraffin: SOCS-4 Antibody [NB100-898]

Immunohistochemistry-Paraffin: SOCS-4 Antibody [NB100-898] - 3.8ug/ml staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for SOCS-4 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary experiments in human and Rodent brain lysates and in lysates of NIH3T3, A431 and HeLa gave no specific signal but low background. IHC-P: Human pancreas shows cytoplasm staining on select beta cells in islet of langerhans.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SOCS-4

The eight members of the recently identified Suppressor of Cytokines Signaling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N- terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS1 and SOCS6 interact with the insulin receptor (IR) when expressed in human hepatoma cells (HepG2) or in rat hepatoma cells overexpressing human IR. SOCS1 and SOCS6 inhibit insulin-dependent activation of ERK1/2 and protein kinase B in vivo and IR- directed phosphorylation of IRS1 in vitro. These results suggest that SOCS proteins may be inhibitors of IR signalling and could mediate cytokine-induced insulin resistance and contribute to the pathogenesis of type II diabetes. SOCS6 and SOCS7 are expressed ubiquitously in murine tissues and SOCS6 knockout mice are growth retarded.

Long Name

Suppressor Of Cytokine Signaling 4

Alternate Names

CIS4, SOCS-7, SOCS4, SSI4

Entrez Gene IDs

122809 (Human)

Gene Symbol

SOCS4

UniProt

NP_543143. (Human)

Additional SOCS-4 Products

Product Documents for SOCS-4 Antibody

Certificate of Analysis

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Product Specific Notices for SOCS-4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SOCS-4 Antibody

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Protocols

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