SOD1/Cu-Zn SOD Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24915

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SOD1/Cu-Zn SOD Primary Antibodies »

Product Specifications

Immunogen

The superoxide dismutase enzyme from bovine erythrocytes (CAS # 9054-89-1) was used as the immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOD1/Cu-Zn SOD Antibody - BSA Free

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Bovine ocular fluid. Antibody at 1:1000. Western blot image submitted by a verified customer review.
Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-24915]

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-24915]

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Analysis of Superoxide Dismutase 1 in FFPE human liver tissue using an isotype control (top) and NBP2-24915 (bottom) at 5 ug/ml.
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Analysis of SOD in liver lysate of 1) human, 2) mouse, and 3) rat using this antibody. 025 ug/ml.
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915]

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Human ocular fluid. Antibody at 1:1000. Western blot image submitted by a verified customer review.
SOD1/Cu-Zn SOD Antibody - BSA Free

Western Blot: SOD1/Cu-Zn SOD Antibody - BSA Free [NBP2-24915] -

Production of superoxide radicals (O2–) and expression of antioxidant enzymes during MSC expansion, with or without methylene blue (MB) addition. a Quantification of superoxide radicals was carried out by MitoSOX staining (n = 3; values represent the mean +/- SEM). *p < 0.05. b Mitochondrial (MnSOD) and cytosolic superoxide dismutase (Cu/ZnSOD) Western blots were performed on proteins isolated from the same samples, and c results were normalized to actin Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28061861), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SOD1/Cu-Zn SOD Antibody - BSA Free

Western Blot: SOD1/Cu-Zn SOD Antibody - BSA Free [NBP2-24915] -

SOD1 does not affect laminin alpha 5 expression in endothelial cells. (A) mRNA levels of human SOD1 in 1G11-mock and 1G11-SOD1 cells, as determined by RT-qPCR. Each dot represents the mean of a triplicate from an independent experiment *** p < 0.001; two-tailed t-test. (B) Human SOD1 protein levels in 1G11-mock and 1G11-SOD1 cells as determined by immunoblotting; HEK-293T cells were used as a human SOD1 reference. Immunoblots were rehybridized with an anti-beta -actin antibody as a loading control. A representative experiment is shown. (C) 1G11-mock and 1G11-SOD1 cells were incubated (14 h) with the conditioned medium from N202.1A tumor cell line cultures (MCT) and then stained with DHE (red); nuclei were DAPI-counterstained (blue). Treatment with the SOD mimetic MnTBAP was used as control (right panels). Scale bars 25 um. (D) SOD activity in 1G11-mock and 1G11-SOD1 cell extracts. (E) Relative LAMA5 mRNA levels in 1G11-mock and 1G11-SOD1 cells. Each dot represents the mean of a triplicate from an independent experiment (n = 5). p = 0.32, two-tailed t-test. (F) Representative images of laminin alpha 5 (green; PAC078MV01) staining in 1G11-mock and 1G11-SOD1 cells; nuclei were DAPI counterstained (blue). Scale bars 50 um. (G) Quantification of laminin alpha 5 mean fluorescence intensity from the images as in (F) (n = 5 fields/condition). p = 0.58; two-tailed t-test. (H) Relative LAMA4 mRNA levels in 1G11-mock, 1G11-SOD3 and 1G11-SOD1 cells. Each dot represents the mean of a triplicate from an independent experiment (n = 6). ** p < 0.01, *** p < 0.001, two-tailed t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35267534), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SOD1/Cu-Zn SOD Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1 - 2 ug/ml

Reviewed Applications

Read 3 reviews rated 5 using NBP2-24915 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOD1/Cu-Zn SOD

FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.

Long Name

Superoxide Dismutase-1

Alternate Names

Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble

Gene Symbol

SOD1

UniProt

P00441

Additional SOD1/Cu-Zn SOD Products

Product Documents for SOD1/Cu-Zn SOD Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SOD1/Cu-Zn SOD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SOD1/Cu-Zn SOD Antibody - BSA Free

Customer Reviews for SOD1/Cu-Zn SOD Antibody - BSA Free (3)

5 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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1 Stars
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  • SOD1/Cu-Zn SOD Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: ocular fluid
    Species: Bovine
    Verified Customer | Posted 09/23/2020
    works really well. Use at a dilution of 1:1000.
    use at a dilution of 1:1000
    SOD1/Cu-Zn SOD Antibody - BSA Free NBP2-24915
  • SOD1/Cu-Zn SOD Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: ocular fluid
    Species: Human
    Verified Customer | Posted 09/23/2020
    works really well at 1:1000 for western blotting
    use at dilution 1:1000
    SOD1/Cu-Zn SOD Antibody - BSA Free NBP2-24915
  • SOD1/Cu-Zn SOD Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver
    Species: Mouse
    Verified Customer | Posted 01/12/2017
    N/A
    SOD1/Cu-Zn SOD Antibody - BSA Free NBP2-24915

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Protocols

View specific protocols for SOD1/Cu-Zn SOD Antibody - BSA Free (NBP2-24915):

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer at all times).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructions.

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FAQs

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