SOD2/Mn-SOD Antibody (10F6) - BSA Free

Novus Biologicals | Catalog # NBP3-26308

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10F6 expressed in HEK293

Format

BSA Free
View all SOD2/Mn-SOD Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human SOD2/Mn-SOD [UniProt P04179]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOD2/Mn-SOD Antibody (10F6) - BSA Free

SOD2/Mn-SOD Antibody (10F6)

Immunohistochemistry: SOD2/Mn-SOD Antibody (10F6) [NBP3-26308] -

Immunohistochemistry: SOD2/Mn-SOD Antibody (10F6) [NBP3-26308] - Image of SOD2/Mn-SOD Antibody (10F6) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
SOD2/Mn-SOD Antibody (10F6)

Western Blot: SOD2/Mn-SOD Antibody (10F6) [NBP3-26308] -

Western Blot: SOD2/Mn-SOD Antibody (10F6) [NBP3-26308] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, Rat brain tissue, Brain tissue, Liver tissue.
All lanes: SOD2/Mn-SOD Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 25, 21, 19, 20 kDa
Observed band size: 25 kDa

Applications for SOD2/Mn-SOD Antibody (10F6) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: SOD2/Mn-SOD

Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32 kDa dimer in the cytosol. Mn SOD is an 80 kDa tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from NFmediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDAand nitric oxide-induced neurotoxicity.

Long Name

Superoxide Dismutase-2

Alternate Names

IPO-B, Mn SOD, MnSOD, SOD, Mitochodrial

Gene Symbol

SOD2

Additional SOD2/Mn-SOD Products

Product Documents for SOD2/Mn-SOD Antibody (10F6) - BSA Free

Certificate of Analysis

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Product Specific Notices for SOD2/Mn-SOD Antibody (10F6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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