Sodium Potassium ATPase Alpha 1 Antibody (ST0533)

Novus Biologicals | Catalog # NBP3-33143

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ST0533 expressed in HEK293
View all Sodium Potassium ATPase Alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Sodium Potassium ATPase Alpha 1 aa 39-83 / 1023. (Uniprot: P05023)

Localization

Cell membrane, Melanosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

113 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sodium Potassium ATPase Alpha 1 Antibody (ST0533)

Sodium Potassium ATPase Alpha 1 Antibody (ST0533)

Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143]

Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33143, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Sodium Potassium ATPase Alpha 1 Antibody (ST0533)

Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143]

Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33143) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Sodium Potassium ATPase Alpha 1 Antibody (ST0533)

Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143]

Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunocytochemistry analysis of HeLa cells labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 Alpha 1 antibody (NBP3-33143) at 1/500 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Sodium Potassium ATPase antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
Sodium Potassium ATPase Alpha 1 Antibody (ST0533)

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143]

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Western blot analysis of Sodium Potassium ATPase Alpha 1 on different lysates with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/100,000 dilution.
Lane 1: HeLa cell lysate (no heat) (20 µg/Lane)
Lane 2: HT-29 cell lysate (no heat) (20 µg/Lane)
Lane 3: HepG2 cell lysate (no heat) (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (no heat) (20 µg/Lane)
Lane 5: L-929 cell lysate (no heat) (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (no heat) (20 µg/Lane)
Lane 7: Rat brain tissue lysate (no heat) (20 µg/Lane)

Predicted band size: 113 kDa
Observed band size: 100 kDa

Exposure time: 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Applications for Sodium Potassium ATPase Alpha 1 Antibody (ST0533)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:200

Immunohistochemistry-Paraffin

1:50-1:5000

Western Blot

1:50000-1:100000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sodium Potassium ATPase Alpha 1

Sodium Potassium ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The Sodium Potassium ATPase enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide (Sodium Potassium ATPase Alpha 1) has been shown to be the catalytically active subunit, whereas the Beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Sodium Potassium ATPase alpha-1 subunit, in the brain, is expressed in both neuronal and non-neuronal cells.

Long Name

Sodium/potassium-transporting ATPase subunit alpha-1

Alternate Names

ATP1A1, EC 7.2.2.13

Gene Symbol

ATP1A1

Additional Sodium Potassium ATPase Alpha 1 Products

Product Documents for Sodium Potassium ATPase Alpha 1 Antibody (ST0533)

Certificate of Analysis

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Product Specific Notices for Sodium Potassium ATPase Alpha 1 Antibody (ST0533)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for Sodium Potassium ATPase Alpha 1 Antibody (ST0533)

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  • Q: I am looking forward to pick up a plasma membrane marker. I have gastric cancer cell line and I am preparing PM out of that. To just check the quality of prep, I need a plasma membrane marker.

    A:

    Our sodium potassium ATPase and plasma membrane products may be of use to you. Please contact us at technical@novusbio.com with any additional questions.

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