Sodium Potassium ATPase Beta 1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-95093
![Western Blot: Sodium Potassium ATPase Beta 1 AntibodyBSA Free [NBP2-95093]](https://resources.rndsystems.com/images/products/Sodium-Potassium-ATPase-Beta-1-Antibody-Western-Blot-NBP2-95093-img0004.jpg "Western Blot: Sodium Potassium ATPase Beta 1 AntibodyBSA Free [NBP2-95093]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 63-303 of human ATP1B1 (NP_001668.1). EFKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIVRFLEKYKDSAQRDDMIFEDCGDVPSEPKERGDFNHERGERKVCRFKLEWLGNCSGLNDETYGYKEGKPCIIIKLNRVLGFKPKPPKNESLETYPVMKYNPNVLPVQCTGKRDEDKDKVGNVEYFGLGNSPGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTMDTEIRIECKAYGENIGYSEKDRFQGRFDVKIEVKS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Sodium Potassium ATPase Beta 1 Antibody - BSA Free
Western Blot: Sodium Potassium ATPase Beta 1 AntibodyBSA Free [NBP2-95093]
Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Analysis of extracts of various cell lines, using Sodium Potassium ATPase Beta 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [NBP2-95093]](https://resources.rndsystems.com/images/products/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0003.jpg "Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [NBP2-95093]")
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [NBP2-95093]
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded mouse stomach using Sodium Potassium ATPase Beta 1.![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [NBP2-95093]](https://resources.rndsystems.com/images/products/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0001.jpg "Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [NBP2-95093]")
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [NBP2-95093]
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded human colon carcinoma using Sodium Potassium ATPase Beta 1.![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [NBP2-95093]](https://resources.rndsystems.com/images/products/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0002.jpg "Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [NBP2-95093]")
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [NBP2-95093]
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded human gastric cancer using Sodium Potassium ATPase Beta 1.
Western Blot: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] -
Western Blot: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Western blot analysis of various lysates using Sodium Potassium ATPase Beta 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] -
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Immunofluorescence analysis of NIH/3T3 cells using Sodium Potassium ATPase Beta 1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] -
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Immunofluorescence analysis of C6 cells using Sodium Potassium ATPase Beta 1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.Applications for Sodium Potassium ATPase Beta 1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Sodium Potassium ATPase Beta 1
Long Name
Sodium/potassium-transporting ATPase subunit beta-1
Alternate Names
ATP1B, ATP1B1
Gene Symbol
ATP1B1
Additional Sodium Potassium ATPase Beta 1 Products
Product Documents for Sodium Potassium ATPase Beta 1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Sodium Potassium ATPase Beta 1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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