Somatostatin R2/SSTR2 Antibody - BSA Free
Novus Biologicals | Catalog # NB300-157
![Immunocytochemistry/ Immunofluorescence: Somatostatin R2/SSTR2 Antibody [NB300-157]](https://resources.rndsystems.com/images/products/Somatostatin-R2-SSTR2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-157-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Somatostatin R2/SSTR2 Antibody [NB300-157]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine
Cited:
Human, Rat, Porcine
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Electron Microscopy
Cited:
Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic peptide made to the C-terminus of rat Somatostatin Receptor 2 [UniProt# P30680]
Reactivity Notes
Porcine reactivity reported in scientific literature (PMID:33002813)
Localization
Cell Membrane
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Somatostatin R2/SSTR2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: Somatostatin R2/SSTR2 Antibody [NB300-157]
Immunocytochemistry/Immunofluorescence: Somatostatin R2/SSTR2 Antibody [NB300-157] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 1:50 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157]](https://resources.rndsystems.com/images/products/Somatostatin-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NB300-157-img0004.jpg "Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157]")
Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157]
Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157] - Staining of Somatostatin Receptor 2 in mouse pancreas using DAB with hematoxylin counterstain.![Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157]](https://resources.rndsystems.com/images/products/Somatostatin-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NB300-157-img0003.jpg "Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157]")
Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157]
Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157] - Staining of paraffin embedded rat pancreas tissue.Applications for Somatostatin R2/SSTR2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:250
Immunohistochemistry
1:100
Immunohistochemistry-Frozen
1:100
Immunohistochemistry-Paraffin
1:100
Immunoprecipitation
1:10 - 1:500
Western Blot
1:100 - 1:1000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Somatostatin R2/SSTR2
Long Name
Somatostatin Receptor Type 2
Alternate Names
Smstr2, SomatostatinR2, SST2, SSTR2
Gene Symbol
SSTR2
UniProt
Additional Somatostatin R2/SSTR2 Products
Product Documents for Somatostatin R2/SSTR2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Somatostatin R2/SSTR2 Antibody - BSA Free
Licensed to Novus Biologicals LLC under U.S. Patent No 5,998,154.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Somatostatin R2/SSTR2 Antibody - BSA Free
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Protocols
View specific protocols for Somatostatin R2/SSTR2 Antibody - BSA Free (NB300-157):
Somatostatin R2/SSTR2 Antibody:
Procedure Guide for NB 300-157 Polyclonal Anti-Somatostatin Receptor II
Immunofluorescence Procedure
1. Plate cells on Lab-TekII chamber slides (Nalge Nunc).
2. Grow cells to about 75% confluence.
3. Rinse cells with PBS, at room temperature (RT).
4. Fix in 2% formaldehyde/PBS for 20 minutes at RT.
5. Rinse the cells 3x with TBS (pH 7.2).
6. Block the non-specific sites with 0.5% normal goat serum in TBS + 0.1% Triton X 100 (TBS-TX).
7. Incubate the cells in anti-SSR2 (catalog# NB 300-157), diluted in TBS-TX, overnight at RT.
8. Next day: rinse the cells 3x 5 minutes, in TBS.
9. Incubate the cells for 1 hour at RT in FITC-goat anti-rabbit secondary, diluted in TBS-TX (1:1,000).
10. Rinse 3x with TBS.
11. Mount the cells in Vector Labs mounting media and coverslip.
Procedure Guide for NB 300-157 Polyclonal Anti-Somatostatin Receptor II
Immunofluorescence Procedure
1. Plate cells on Lab-TekII chamber slides (Nalge Nunc).
2. Grow cells to about 75% confluence.
3. Rinse cells with PBS, at room temperature (RT).
4. Fix in 2% formaldehyde/PBS for 20 minutes at RT.
5. Rinse the cells 3x with TBS (pH 7.2).
6. Block the non-specific sites with 0.5% normal goat serum in TBS + 0.1% Triton X 100 (TBS-TX).
7. Incubate the cells in anti-SSR2 (catalog# NB 300-157), diluted in TBS-TX, overnight at RT.
8. Next day: rinse the cells 3x 5 minutes, in TBS.
9. Incubate the cells for 1 hour at RT in FITC-goat anti-rabbit secondary, diluted in TBS-TX (1:1,000).
10. Rinse 3x with TBS.
11. Mount the cells in Vector Labs mounting media and coverslip.
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Somatostatin R2/SSTR2 Antibody - BSA Free
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Q: Is there any data to show that this antibody specifically detects sstr2 since it says that it is unpurified sera?
A:
All of our current data for NB300-157 is available online. We have 6 available publications for this antibody as well. This product has been shown to specifically target the protein of interest by WB in published data. If you are interested in a blocking peptide to assist in your research, we do offer one specifically for this antibody - Somatostatin R2 SSTR2 Peptide (NB300-157PEP).
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