Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74288

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI9E7

Format

Azide and BSA Free
View all Sorbitol Dehydrogenase Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SORD (NP_003095) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

38.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free

Western Blot: Sorbitol Dehydrogenase Antibody (OTI9E7)Azide and BSA Free [NBP2-74288]

Western Blot: Sorbitol Dehydrogenase Antibody (OTI9E7)Azide and BSA Free [NBP2-74288]

Western Blot: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Sorbitol Dehydrogenase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti
Immunocytochemistry/ Immunofluorescence: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288]

Immunocytochemistry/ Immunofluorescence: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288]

Immunocytochemistry/Immunofluorescence: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288] - Staining of HepG2 cells using anti-SORD mouse monoclonal antibody.
Flow Cytometry: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288]

Flow Cytometry: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288]

Flow Cytometry: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288] - HEK293T cells transfected with either pCMV6-ENTRY Sorbitol Dehydrogenase.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-Sorbitol Dehydrogenase mouse monoclonal, and then analyzed by flow cytometry.
Western Blot: Sorbitol Dehydrogenase Antibody (OTI9E7)Azide and BSA Free [NBP2-74288]

Western Blot: Sorbitol Dehydrogenase Antibody (OTI9E7)Azide and BSA Free [NBP2-74288]

Western Blot: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Sorbitol Dehydrogenase monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288]

Immunocytochemistry/ Immunofluorescence: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288]

Immunocytochemistry/Immunofluorescence: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Sorbitol Dehydrogenase.
Flow Cytometry: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288]

Flow Cytometry: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288]

Flow Cytometry: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288] - Analysis of Hela cells, using anti-Sorbitol Dehydrogenase antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288]

Flow Cytometry: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288]

Flow Cytometry: Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free [NBP2-74288] - Analysis of Jurkat cells, using anti-Sorbitol Dehydrogenase antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Applications for Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sorbitol Dehydrogenase

Sorbitol dehydrogenase (SDH), a member of the medium-chain dehydrogenase/reductase protein family and the second enzyme of the polyol pathway of glucose metabolism, converts sorbitol to fructose strictly using NAD(+) as coenzyme. SDH is expressed almost ubiquitously in all mammalian tissues. The enzyme has attracted considerable interest due to its implication in the development of diabetic complications as the polyol pathway is particularly active in hyperglycemic states. Although SORD is closely related to the class I long-chain alcohol dehydrogenases, it differs in substrate specificity, catalyzing polyols such as sorbitol and xylitol but having no activity towards primary alcohols.

Alternate Names

EC 1.1.1, EC 1.1.1.14, L-iditol 2-dehydrogenase, sorbitol dehydrogenase, SORD1

Gene Symbol

SORD

Additional Sorbitol Dehydrogenase Products

Product Documents for Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Sorbitol Dehydrogenase Antibody (OTI9E7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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