SOX10 Antibody (CL4455) - BSA Free

Novus Biologicals | Catalog # NBP2-59050

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Feline

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL4455

Format

BSA Free
View all SOX10 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P56693, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Feline reactivity reported from a verified customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SOX10 Antibody (CL4455) - BSA Free

Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (CL4455) [NBP2-59050]

Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (CL4455) [NBP2-59050]

Immunocytochemistry/Immunofluorescence: SOX10 Antibody (CL4455) [NBP2-59050] - staining of SK-MEL-30 cells using the Anti-SOX10 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Frozen: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Frozen: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Frozen: SOX10 Antibody (CL4455) [NBP2-59050] - Feline adult spinal cord demonstrating SOX10 (red) and DAPI (blue). Antibody at 1:200 dilutiion in 1X PBS + 0.05% tween 20+ 2% BSA. Tested at 1:50, 1:100, 1:200, 1:500, and 1:1000. Dilution of 1:200 gave the best signal/noise ratio for IHC-Fr. The slides were subsequently incubated with Alexa Fluor conjugated secondary antibody for 1hr at RT and imaged with fluorescent microscopy. Image submitted by a verified customer review.
Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing trigeminal ganglion and ear placode.
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cerebral cortex shows strong nuclear immunoreactivity in a subset of glial cells.
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human breast shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cervix shows nuclear immunoreactivity in a subset of basal cells in squamous epithelium.
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human pancreas shows absence of positivity as expected (negative control).

Applications for SOX10 Antibody (CL4455) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 3 using NBP2-59050 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX10

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

Long Name

SRY-box transcription factor 10

Alternate Names

DOM, PCWH, SOX-10, WS2E, WS4, WS4C

Gene Symbol

SOX10

Additional SOX10 Products

Product Documents for SOX10 Antibody (CL4455) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SOX10 Antibody (CL4455) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SOX10 Antibody (CL4455) - BSA Free (1)

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Customer Images

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  • SOX10 Antibody (CL4455)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Adult spinal cord
    Species: Feline
    Verified Customer | Posted 03/14/2019
    Image was captured using fluorescent microscopy demonstrating SOX10 (red) and DAPI (blue).
    For NBP2-59050 antibody, I used it at 1:200 in 1X PBS + 0.05% tween 20+ 2% BSA. As I tested it at 1:50, 1:100, 1:200, 1:500 and 1:1000, that concentration gave the best signal/noise ratio for IHC-Fr. The slides were subsequently incubated with alexa fluor conjugated secondary antibody for 1hr at RT and imaged with fluorescent microscopy.
    SOX10 Antibody (CL4455) - BSA Free NBP2-59050

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Protocols

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FAQs

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