SOX21 Antibody (CL4688) - BSA Free

Novus Biologicals | Catalog # NBP2-59059

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL4688

Format

BSA Free
View all SOX21 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from Q9Y651, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SOX21 Antibody (CL4688) - BSA Free

Western Blot: SOX21 Antibody (CL4688) [NBP2-59059]

Western Blot: SOX21 Antibody (CL4688) [NBP2-59059]

Western Blot: SOX21 Antibody (CL4688) [NBP2-59059] - Analysis in human cell line NTERA-2.
Immunocytochemistry/ Immunofluorescence: SOX21 Antibody (CL4688) [NBP2-59059]

Immunocytochemistry/ Immunofluorescence: SOX21 Antibody (CL4688) [NBP2-59059]

Immunocytochemistry/Immunofluorescence: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of PC-3 cells using the Anti-SOX21 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059]

Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059]

Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of human fallopian tube shows no positivity as expected (negative control).
Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059]

Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059]

Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of mouse embryo E14 shows nuclear immunoreactivity in the developing forebrain.
Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059]

Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059]

Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of mouse forebrain shows nuclear immunoreactivity in a subset of cells in the lateral ventricle wall.
Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059]

Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059]

Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of mouse cerebral cortex shows nuclear positivity in a subset of cells.
Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059]

Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059]

Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of human stomach shows moderate nuclear positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059]

Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059]

Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of human cervix shows moderate nuclear immunoreactivity in a subset of squamous epithelial cells.

Applications for SOX21 Antibody (CL4688) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX21

SOX21 - SRY (sex determining region Y)-box 21

Long Name

Transcription Factor SOX21

Alternate Names

SOX25

Gene Symbol

SOX21

Additional SOX21 Products

Product Documents for SOX21 Antibody (CL4688) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SOX21 Antibody (CL4688) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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