SOX6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86149

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SOX6 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QATSPFACAADGEDAMTQDLTSREKEEGSDQHVASHLPLHPIMHNKPHSEELPTLVSTIQQDADWDSVLSSQQRMESENNKLCSLYSFRNTSTSPHKPDEGSRDREIMTSVTFGTPERRKGSLADVVDTLKQKKLEEMTRTEQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOX6 Antibody - BSA Free

Western Blot: SOX6 Antibody [NBP1-86149]

Western Blot: SOX6 Antibody [NBP1-86149]

Western Blot: SOX6 Antibody [NBP1-86149] - Analysis in control (vector only transfected HEK293T lysate) and SOX6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
SOX6 Antibody - BSA Free Immunohistochemistry-Paraffin: SOX6 Antibody - BSA Free [NBP1-86149]

Immunohistochemistry-Paraffin: SOX6 Antibody - BSA Free [NBP1-86149]

Immunohistochemistry analysis in human testis and endometrium tissues using HPA003908 antibody. Corresponding SOX6 RNA-seq data are presented for the same tissues.
SOX6 Antibody - BSA Free Immunohistochemistry-Paraffin: SOX6 Antibody - BSA Free [NBP1-86149]

Immunohistochemistry-Paraffin: SOX6 Antibody - BSA Free [NBP1-86149]

Staining of human cerebral cortex shows moderate nuclear positivity in neurons.
SOX6 Antibody - BSA Free Immunohistochemistry-Paraffin: SOX6 Antibody - BSA Free [NBP1-86149]

Immunohistochemistry-Paraffin: SOX6 Antibody - BSA Free [NBP1-86149]

Staining of human testis shows strong nuclear positivity in spermatids.
SOX6 Antibody - BSA Free Immunohistochemistry-Paraffin: SOX6 Antibody - BSA Free [NBP1-86149]

Immunohistochemistry-Paraffin: SOX6 Antibody - BSA Free [NBP1-86149]

Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.
SOX6 Antibody - BSA Free Immunohistochemistry-Paraffin: SOX6 Antibody - BSA Free [NBP1-86149]

Immunohistochemistry-Paraffin: SOX6 Antibody - BSA Free [NBP1-86149]

Staining of human endometrium shows no positivity in glandular cells as expected.
SOX6 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: SOX6 Antibody - BSA Free [NBP1-86149]

Chromatin Immunoprecipitation-exo-Seq: SOX6 Antibody - BSA Free [NBP1-86149]

ChIP-Exo-Seq composite graph for Anti-SOX6 (NBP1-86149) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for SOX6 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX6

Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.

Long Name

SRY-related HMG-box 6

Alternate Names

SOXD

Gene Symbol

SOX6

Additional SOX6 Products

Product Documents for SOX6 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SOX6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Chondrogenesis Markers

Citations for SOX6 Antibody - BSA Free

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Protocols

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