Catalog Number: 1496
Chemical Name: Anthra[1-9-cd]pyrazol-6(2H)-one
Biological Activity
Selective JNK inhibitor. Competitively and reversibly inhibits JNK1, 2 and 3 (IC50 = 40 - 90 nM) with negligible activity at ERK2, p38β and a range of enzymes. Active in vivo. Exhibits reduced selectivity over other protein kinases under certain conditions. Protects renal tubular epithelial cells against ischemia/reperfusion-induced apoptosis. Prevents BMP9-induced osteogenic differentiation of MSCs. Essential component of medium for maintaining stem cells in naive pluripotent state. Available as part of the MAPK Inhibitor Tocriset™.
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 5 mM in ethanol with gentle warming and to 100 mM in DMSO
  • Purity:
  • Storage:
    Desiccate at -20°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase.
    Bennett et al.
    Proc.Natl.Acad.Sci.U.S.A., 2001;98:13681
  2. TAK1/JNK and p38 have opposite effects on rat hepatic stellate cells.
    Schnabl et al.
    Hepatology, 2001;34:953
  3. SP600125, a selective JNK inhibitor, protects ischemic renal injury via suppressing the extrinsic pathways of apoptosis.
    Wang et al.
    Life Sci., 2007;80:2067
  4. Activation of JNKs is essential for BMP9-induced osteogenic differentiation of mesenchymal stem cells.
    Zhao et al.
    BMB Rep., 2013;46:422
  5. Derivation of novel human ground state naive pluripotent stem cells.
    Gafni et al.
    Nature, 2013;504:282

The citations listed below are publications that use Tocris products. Selected citations for SP 600125 include:

Showing Results 1 - 10 of 69

  1. 18α-glycyrrhetinic acid lethality for neuroblastoma cells via de-regulating the Beclin-1/Bcl-2 complex and inducing apoptosis.
    Authors: Rahman Et al.
    Biochemical Pharmacology
  2. Effects of jnk inhibitor on inflammation and fibrosis in the ovary tissue of a rat model of polycystic ovary syndrome.
    Authors: Bulut Et al.
    Exp Hematol Oncol
  3. Hyperphosphate-Induced Myocardial Hypertrophy through the GATA-4/NFAT-3 Signaling Pathway Is Attenuated by ERK Inhibitor Treatment.
    Authors: Liu Et al.
    Int J Mol Sci
  4. Tissue factor/factor VIIa signalling promotes cytokine-induced beta cell death and impairs glucose-stimulated insulin secretion from human pancreatic islets.
    Authors: Edén Et al.
  5. Analysis of the anti-proliferative and the pro-apoptotic efficacy of Syk inhibition in multiple myeloma.
    Authors: Koerber Et al.
    Nat Commun
  6. Free fatty acids shift insulin-induced hepatocyte proliferation towards CD95-dependent apoptosis.
    Authors: Sommerfeld Et al.
    J Biomed Sci
  7. Black Rice Anthocyanins Suppress Metastasis of Breast Cancer Cells by Targeting RAS/RAF/MAPK Pathway.
    Authors: Chen Et al.
    Int J Clin Exp Pathol
  8. Inhibition of G9a Histone Methyltransferase Converts Bone Marrow Mesenchymal Stem Cells to Cardiac Competent Progenitors.
    Authors: Yang Et al.
    PLoS One
  9. Neutrophil IL-1β processing induced by pneumolysin is mediated by the NLRP3/ASC inflammasome and caspase-1 activation and is dependent on K+ efflux.
    Authors: Karmakar Et al.
    Nat Commun
  10. Inhibitory effect of statins on inflammation-related pathways in human abdominal aortic aneurysm tissue.
    Authors: Yoshimura Et al.
    Biomed Res Int
  11. Activation of transcription factor AP-1 in response to thermal injury in rat small intestine and IEC-6 cells.
    Authors: Zhang Et al.
    BMC Gastroenterol
  12. The Effects of the Endocannabinoids Anandamide and 2-Arachidonoylglycerol on Human Osteoblast Proliferation and Differentiation.
    Authors: Smith Et al.
    J Biol Chem
  13. Adiponectin ameliorates experimental periodontitis in diet-induced obesity mice.
    Authors: Zhang Et al.
    PLoS One
  14. Differential effects of the Toll-like receptor 2 agonists, PGN and Pam3CSK4 on anti-IgE induced human mast cell activation.
    Authors: Yu Et al.
    PLoS One
  15. Angiogenic growth factors augment K-Cl cotransporter expression in erythroid cells via hypoxia-inducible factor-1α.
    Authors: Gonsalves Et al.
    Am J Hematol
  16. Endocannabinoids modulate cortical development by configuring Slit2/Robo1 signalling.
    Authors: Alpár Et al.
  17. miR-155 induced transcriptome changes in the MCF-7 breast cancer cell line leads to enhanced mitogen activated protein kinase signaling.
    Authors: Martin Et al.
    Korean J Physiol Pharmacol
  18. AMP-activated protein kinase determines apoptotic sensitivity of cancer cells to ginsenoside-Rh2.
    Authors: Kim Et al.
    J Ginseng Res
  19. P2Y2 nucleotide receptor activation enhances the aggregation and self-organization of dispersed salivary epithelial cells.
    Authors: El-Sayed Et al.
    J Cell Mol Med
  20. Activation of pattern recognition receptors in brown adipocytes induces inflammation and suppresses uncoupling protein 1 expression and mitochondrial respiration.
    Authors: Bae Et al.
    Am J Physiol Cell Physiol
  21. Decrease of PKB/Akt Phosphorylation is Partially Mediated by SAPK/JNK Activation in Serum-free L6 Myoblasts Starved with Low Glucose.
    Authors: Kim Et al.
    J Phys Ther Sci
  22. Protective effect of Danggui (Radix Angelicae Sinensis) on angiotensin II-induced apoptosis in H9c2 cardiomyoblast cells.
    Authors: Huang Et al.
    BMC Complement Altern Med
  23. Suppression of MAPK/JNK-MTORC1 signaling leads to premature loss of organelles and nuclei by autophagy during terminal differentiation of lens fiber cells.
    Authors: Basu Et al.
  24. Transcriptional regulation of an insulin-sensitizing adipokine adipolin/CTRP12 in adipocytes by Krüppel-like factor 15.
    Authors: Enomoto Et al.
    Am J Physiol Lung Cell Mol Physiol
  25. Sphingosine 1-phosphate counteracts the effects of interleukin-1β in human chondrocytes.
    Authors: Stradner Et al.
    Genes Cancer
  26. Arginase-II induces vascular smooth muscle cell senescence and apoptosis through p66Shc and p53 independently of its l-arginine ureahydrolase activity: implications for atherosclerotic plaque vulnerability.
    Authors: Xiong Et al.
    J Am Heart Assoc
  27. Identification and characterization of a novel class of c-Jun N-terminal kinase inhibitors.
    Authors: Schepetkin Et al.
    Mol Pharmacol
  28. Desipramine protects neuronal cell death and induces heme oxygenase-1 expression in Mes23.5 dopaminergic neurons.
    Authors: Lin Et al.
    PLoS One
  29. Free fatty acid palmitate impairs the vitality and function of cultured human bladder smooth muscle cells.
    Authors: Oberbach Et al.
    Am J Physiol Cell Physiol
  30. Activation of canonical Wnt signalling is required for TGF-β-mediated fibrosis.
    Authors: Akhmetshina Et al.
    Stem Cells Int
  31. Fibroblast growth factor-23 abolishes 1,25-dihydroxyvitamin D3-enhanced duodenal calcium transport in male mice.
    Authors: Khuituan Et al.
    Am J Physiol Endocrinol Metab
  32. Cyclic AMP increases COX-2 expression via mitogen-activated kinase in human myometrial cells.
    Authors: Chen Et al.
    PLoS One
  33. Inflammatory muscle pain is dependent on the activation of kinin B1 and B2 receptors and intracellular kinase pathways.
    Authors: Meotti Et al.
    Br J Pharmacol
  34. Mitochondrial localized STAT3 is involved in NGF induced neurite outgrowth.
    Authors: Zhou and Too
    Arthritis Rheum
  35. Reversible differentiation of myofibroblasts by MyoD.
    Authors: Hecker Et al.
    J Biol Chem
  36. Dermatophagoides pteronyssinus 2 regulates nerve growth factor release to induce airway inflammation via a reactive oxygen species-dependent pathway.
    Authors: Ye Et al.
    J Inflamm (Lond)
  37. JNK suppression is essential for 17β-Estradiol inhibits prostaglandin E2-Induced uPA and MMP-9 expressions and cell migration in human LoVo colon cancer cells.
    Authors: Hsu Et al.
    PLoS One
  38. MUC5AC expression through bidirectional communication of Notch and epidermal growth factor receptor pathways.
    Authors: Kang Et al.
    J Immunol
  39. Thrombin-induced Migration and Matrix Metalloproteinase-9 Expression Are Regulated by MAPK and PI3K Pathways in C6 Glioma Cells.
    Authors: Kim Et al.
    Nat Commun
  40. Phosphorylation of Junb family proteins by the Jun N-terminal kinase supports tissue regeneration in zebrafish.
    Authors: Ishida Et al.
    Dev Biol
  41. Prevention of premature senescence requires JNK regulation of Bcl-2 and reactive oxygen species.
    Authors: Lee Et al.
  42. miR-155 and its star-form partner miR-155* cooperatively regulate type I interferon production by human plasmacytoid dendritic cells.
    Authors: Zhou Et al.
  43. c-Jun protects hypoxia-inducible factor-1alpha from degradation via its oxygen-dependent degradation domain in a nontranscriptional manner.
    Authors: Yu Et al.
    Cancer Res
  44. AMP kinase signaling determines whether c-Jun N-terminal kinase promotes survival or apoptosis during glucose deprivation.
    Authors: Yun Et al.
  45. Ethanol augments RANTES/CCL5 expression in rat liver sinusoidal endothelial cells and human endothelial cells via activation of NF-kappa B, HIF-1 alpha, and AP-1.
    Authors: Yeligar Et al.
    J Immunol
  46. The expression of the beta-defensins hBD-2 and hBD-3 is differentially regulated by NF-kappaB and MAPK/AP-1 pathways in an in vitro model of Candida esophagitis.
    Authors: Steubesand Et al.
    BMC Immunol
  47. 15-deoxy-Delta12,14-prostaglandin J2 up-regulates death receptor 5 gene expression in HCT116 cells: involvement of reactive oxygen species and C/EBP homologous transcription factor gene transcription.
    Authors: Su Et al.
    Mol Cancer Ther
  48. Glioma regression in vitro and in vivo by a suicide combined treatment.
    Authors: García-Escudero Et al.
    Exp Cell Res
  49. Reactive oxygen species stabilize hypoxia-inducible factor-1 alpha protein and stimulate transcriptional activity via AMP-activated protein kinase in DU145 human prostate cancer cells.
    Authors: Jung Et al.
  50. MAP kinases mediate interleukin-13 effects on calcium signaling in human airway smooth muscle cells.
    Authors: Moynihan Et al.
    Am J Physiol Lung Cell Mol Physiol
  51. c-Jun controls histone modifications, NF-kappaB recruitment, and RNA polymerase II function to activate the ccl2 gene.
    Authors: Wolter Et al.
    Mol Cell Biol
  52. Transcriptional regulation of EGR-1 by the interleukin-1-JNK-MKK7-c-Jun pathway.
    Authors: Hoffmann Et al.
    J Biol Chem
  53. Ceramide induces p38 MAPK and JNK activation through a mechanism involving a thioredoxin-interacting protein-mediated pathway.
    Authors: Chen Et al.
  54. Delayed and persistent ERK1/2 activation is required for 4-hydroxytamoxifen-induced cell death.
    Authors: Zhou Et al.
    Mol Cancer Res
  55. 5-Aminoimidazole-4-carboxamide riboside sensitizes TRAIL- and TNF{alpha}-induced cytotoxicity in colon cancer cells through AMP-activated protein kinase signaling.
    Authors: Su Et al.
    Mol Cancer Ther
  56. Paclitaxel-induced nuclear translocation of FOXO3a in breast cancer cells is mediated by c-Jun NH2-terminal kinase and Akt.
    Authors: Sunters Et al.
    Cancer Res
  57. Different roles for non-receptor tyrosine kinases in arachidonate release induced by zymosan and Staphylococcus aureus in macrophages.
    Authors: Olsson and Sundler
    J Immunol
  58. Paclitaxel (Taxol) upregulates expression of functional interleukin-6 in human ovarian cancer cells through multiple signaling pathways.
    Authors: Wang Et al.
  59. Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA.
    Authors: Guillemot and Citi
    Mol Biol Cell
  60. Two distinct signaling pathways regulate peroxynitrite-induced apoptosis in PC12 cells.
    Authors: Shacka Et al.
    Cardiorenal Med
  61. Transforming growth factor-beta1 regulates macrophage migration via RhoA.
    Authors: Kim Et al.
  62. Direct phosphorylation and regulation of poly(ADP-ribose) polymerase-1 by extracellular signal-regulated kinases 1/2.
    Authors: Kauppinen Et al.
    Proc Natl Acad Sci U S A
  63. Estrogen receptor alpha pathway is involved in the regulation of Calbindin-D9k in the uterus of immature rats.
    Authors: Lee Et al.
    Toxicol Sci
  64. Glucose deprivation increases mRNA stability of vascular endothelial growth factor through activation of AMP-activated protein kinase in DU145 prostate carcinoma.
    Authors: Yun Et al.
  65. MEK1-dependent delayed expression of Fos-related antigen-1 counteracts c-Fos and p65 NF-kappaB-mediated interleukin-8 transcription in response to cytokines or growth factors.
    Authors: Hoffmann Et al.
    J Biol Chem
  66. Oxygen-sensitive {δ}-opioid receptor-regulated survival and death signals: novel insights into neuronal preconditioning and protection.
    Authors: Ma Et al.
    PLoS One
  67. Disruption of the c-JUN-JNK complex by a cell-permeable peptide containing the c-JUN delta domain induces apoptosis and affects a distinct set of interleukin-1-induced inflammatory genes.
    Authors: Holzberg Et al.
    J Biol Chem
  68. Mechanisms in LPA-induced tumor cell migration: critical role of phosphorylated ERK.
    Authors: Stähle Et al.
    J Cell Sci
  69. Acadesine activates AMPK and induces apoptosis in B-cell chronic lymphocytic leukemia cells but not in T lymphocytes.
    Authors: Campàs Et al.
    Cell Death Differ
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