SP 600125

Tocris Bioscience | Catalog # 1496

Selective JNK inhibitor
Tocris Bioscience

Key Product Details

Description

Selective JNK inhibitor
View all JNK/c-jun Inhibitors »

Product Description

SP 600125 is a selective JNK inhibitor. Competitively and reversibly inhibits JNK1, 2 and 3 (IC50 = 40 - 90 nM) with negligible activity at ERK2, p38β and a range of enzymes. Active in vivo. Exhibits reduced selectivity over other protein kinases under certain conditions. SP 600125 protects renal tubular epithelial cells from ischemia/reperfusion-induced apoptosis. Promotes chondrogenesis of bone mesenchymal stem cells by inhibition of TNF-α-induced inflammation. Adipogenesis from MSCs is also promoted by SP 600125. Essential component of medium for maintaining stem cells in naive pluripotent state.

Product Specifications for SP 600125

Molecular Weight

220.23

Formula

C14H8N2O

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

Anthra[1-9-cd]pyrazol-6(2H)-one

CAS Number

129-56-6

PubChem ID

8515

InChI Key

ACPOUJIDANTYHO-UHFFFAOYSA-N

SMILES

O=C1C2=C3C(NN=C3C3=CC=CC=C13)=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 22.02 100

Preparing Stock Solutions for SP 600125

The following data is based on the product molecular weight 220.23.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.54 mL 22.70 mL 45.41 mL
5 mM 0.91 mL 4.54 mL 9.08 mL
10 mM 0.45 mL 2.27 mL 4.54 mL
50 mM 0.09 mL 0.45 mL 0.91 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 459 publications citing the usage of this product.

Product Documents for SP 600125

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SP 600125

For research use only

Customer Reviews for SP 600125 (4)

4.8 out of 5
4 Customer Ratings
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  • Role of JNK activation in chemotherapy-induced apoptosis
    Name: MARINA KOUTSIOUMPA
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 05/31/2021
    Human cancer cells were pretreated for 2 hours with SP600125 before addition of chemotherapeutics and were incubated for 72 hours. SP600125 was used at 1, 3 and 10 μM. Real time apoptosis assays were performed. JNK inhibition partially reversed the chemotherapeutics-induced cellular apoptosis.
  • JNK inhibition in LPA induced pro-inflammatory phenotype in microglia.
    Name: lisha joshi
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Primary murine microglia cells
    Verified Customer | Posted 06/10/2019
    Primary murine microglia were treated with LPA (1µM) in the presence or absence of SP600125 (10µM) in order to investigate the role of JNK pathway in the LPA induced inflammatory phenotype. The inhibitor was well tolerated by the cells. DMSO was used as a vehicle control. Cells were lysed and collected for WB analysis in order to identify changes in the expression of COX2 and Arg1.
    SP 600125 1496
  • SP 600125 inhibits JNK activation in HOCl-LDL treated cardiomyocytes
    Name: Chintan Koyani
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: HL-1 cardiomyocytes
    Verified Customer | Posted 10/13/2018
    HL-1 cardiomyocytes were treated with SP 600125 (5 or 10 μM, 30 min) followed by 500 μg/ml HOCl-LDL (oxidant:lipoprotein molar ratio=800:1) for 1 h to follow pJNK expression using Western blot. SP 600125 at 10 μM concentration, completely inhibited activation of JNK.
    SP 600125 1496
  • JNK inhibition in BV2 microglia
    Name: Joanna Plastira
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: BV2 microglia
    Verified Customer | Posted 10/10/2018
    The inhibitor was used at a concentration of 10µM in BV2 microglia cell line. SP600125 inhibited the LPA induced activation of transcription factors (western blotting). It was also used in flow cytometry, ELISAs, IF and functional assays. The results were constant and reproducible.
    SP 600125 1496

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