SP-B/Surfactant Protein B Antibody

Novus Biologicals | Catalog # NBP3-11280

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Porcine, Bovine

Applications

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoassay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
View all SP-B/Surfactant Protein B Primary Antibodies »

Product Specifications

Immunogen

The polyclonal antibody is raised against human-based 60-amino acid synthetic SP-B polypeptide.

Clonality

Polyclonal

Host

Rabbit

Applications for SP-B/Surfactant Protein B Antibody

Application
Recommended Usage

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Immunohistochemistry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SP-B/Surfactant Protein B

The polyclonal antibody recognizes the human surfactant protein B (SP-B). There are four surfactant-specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant. SP-B is synthesized by the alveolar type II epithelial cells as a 40-42 kD precursor that is subsequently proteolytically processed to 7.8-8 kD. SP-B enhances the spreading and stability of surfactant phospholipids in the alveolus. SP-B is essential for air-breathing in mammals and is therefore largely conserved. SP-B can interact with both phospholipid head groups and fatty chains and is particularly active in enhancing surface active behaviour in endogenous and exogenous lung surfactants. Even low SP-B contents had measurable effects in increasing the adsorption, dynamic surface tension lowering, and/or film respreading of DPPC, mixed synthetic lipids, and column-purified lung surfactant phospholipids. Deficiency of SP-B and other surfactant components is associated with respiratory distress syndrome (RDS) in premature infants and adults with respiratory distress syndrome (ARDS). The polyclonal antibody is raised against human-based 60-amino acid synthetic SP-B polypeptide. The antibody recognizes proteins of 42, 25 and 18 kD under nonreducing conditions. The 18 kD form is reduced to 7.7-8 kD in the presence of beta-mercaptoethanol. The polyclonal antibody is cross reactive with mouse, bovine and pig SP-B.

Long Name

Surfactant Protein B

Alternate Names

PSP-B, SFTB3, SFTP3, SFTPB, SMDP1

Gene Symbol

SFTPB

Additional SP-B/Surfactant Protein B Products

Product Documents for SP-B/Surfactant Protein B Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for SP-B/Surfactant Protein B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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