SP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89458

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NGGGAFSQARSSSTGSSSSTGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTNGSNGSESSKNRTVSG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SP1 Antibody - BSA Free

SP1 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: SP1 Antibody - BSA Free [NBP1-89458]

Immunocytochemistry/ Immunofluorescence: SP1 Antibody - BSA Free [NBP1-89458]

Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458]

Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458]

Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458]

Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458]

Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458]

Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458]

Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458]

Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458]

Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes.
SP1 Antibody - BSA Free Western Blot: SP1 Antibody - BSA Free [NBP1-89458]

Western Blot: SP1 Antibody - BSA Free [NBP1-89458]

Analysis using Anti-SP1 antibody NBP1-89458 (A) shows similar pattern to independent antibody NBP1-85812 (B).

Applications for SP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SP1

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling

Alternate Names

Sp1 transcription factor, specificity protein 1, transcription factor Sp1, TSFP1

Gene Symbol

SP1

Additional SP1 Products

Product Documents for SP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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