SP110 Antibody (8C8) - Azide and BSA Free

Novus Biologicals | Catalog # H00003431-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 8C8

Format

Azide and BSA Free
View all SP110 Primary Antibodies »

Product Specifications

Immunogen

SP110 (NP_004501, 271 a.a. ~ 380 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TPSDKKGKKRKRCIWSTPKRRHKKKSLPRGTASSRHGIQKKLKRVDQVPQKKDDSTCNSTVETRAQKARTECARKSRSEEIIDGTSEMNEGKRSQKTPSTPRRVTQGAAS

Specificity

SP110 (8C8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SP110 Antibody (8C8) - Azide and BSA Free

Western Blot: SP110 Antibody (8C8) [H00003431-M01]

Western Blot: SP110 Antibody (8C8) [H00003431-M01]

Western Blot: SP110 Antibody (8C8) [H00003431-M01] - Analysis of SP110 expression in transfected 293T cell line by SP110 monoclonal antibody (M01), clone 8C8.Lane 1: SP110 transfected lysate(61.8 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: SP110 Antibody (8C8) [H00003431-M01]

Immunohistochemistry-Paraffin: SP110 Antibody (8C8) [H00003431-M01]

Immunohistochemistry-Paraffin: SP110 Antibody (8C8) [H00003431-M01] - Analysis of monoclonal antibody to SP110 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Western Blot: SP110 Antibody (8C8) [H00003431-M01]

Western Blot: SP110 Antibody (8C8) [H00003431-M01]

Western Blot: SP110 Antibody (8C8) [H00003431-M01] - SP110 expression in Jurkat ( Cat # L017V1 ).
Western Blot: SP110 Antibody (8C8) [H00003431-M01]

Western Blot: SP110 Antibody (8C8) [H00003431-M01]

Western Blot: SP110 Antibody (8C8) [H00003431-M01] - Analysis of SP110 over-expressed 293 cell line, cotransfected with SP110 Validated Chimera RNAi ( Cat # H00003431-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SP110 monoclonal antibody (M01), clone 8C8 (Cat # H00003431-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunoprecipitation: SP110 Antibody (8C8) [H00003431-M01]

Immunoprecipitation: SP110 Antibody (8C8) [H00003431-M01]

Immunoprecipitation: SP110 Antibody (8C8) [H00003431-M01] - Analysis of SP110 transfected lysate using anti-SP110 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SP110 MaxPab rabbit polyclonal antibody.
ELISA: SP110 Antibody (8C8) [H00003431-M01]

ELISA: SP110 Antibody (8C8) [H00003431-M01]

ELISA: SP110 Antibody (8C8) [H00003431-M01] - Detection limit for recombinant GST tagged SP110 is approximately 0.1ng/ml as a capture antibody.

Applications for SP110 Antibody (8C8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SP110

The nuclear body is a multiprotein complex that may have a role in the regulation of gene transcription. This gene is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. In addition, it has been suggested that the protein may play a role in ribosome biogenesis and in the induction of myeloid cell differentiation. Alternative splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified.

Alternate Names

FLJ22835, IFI41, IFI75, interferon-induced protein 41, 30kD, Interferon-induced protein 41/75, interferon-induced protein 75, 52kD, IPR1, phosphoprotein 41, phosphoprotein 75, SP110 nuclear body protein, Speckled 110 kDa, Transcriptional coactivator Sp110, VODI

Entrez Gene IDs

3431 (Human); 109032 (Mouse); 301570 (Rat)

Gene Symbol

SP110

OMIM

604457 (Human)

UniProt

NP_004501 (Human)

Additional SP110 Products

Product Documents for SP110 Antibody (8C8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SP110 Antibody (8C8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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