SP6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92428

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ESDSPLAPGPFSKLLQPDMSHHYESWFRPTHPGAEDGSWWDLHPGTSWMDLPHTQGALTSPGHPGALQAGLGGYVGDHQLCAP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SP6 Antibody - BSA Free

Western Blot: SP6 Antibody [NBP1-92428]

Western Blot: SP6 Antibody [NBP1-92428]

Western Blot: SP6 Antibody [NBP1-92428] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: SP6 Antibody [NBP1-92428]

Immunocytochemistry/ Immunofluorescence: SP6 Antibody [NBP1-92428]

Immunocytochemistry/Immunofluorescence: SP6 Antibody [NBP1-92428] - Staining of human cell line CACO-2 shows localization to nucleoplasm, centrosome & mitotic spindle. Antibody staining is shown in green.
SP6 Antibody - BSA Free Western Blot: SP6 Antibody - BSA Free [NBP1-92428]

Western Blot: SP6 Antibody - BSA Free [NBP1-92428]

Analysis in control (vector only transfected HEK293T lysate) and SP6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for SP6 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF,Recommended conditions Fixation/Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SP6

SP6 - Sp6 transcription factor

Alternate Names

Epfn, EPIPROFIN, KLF14MGC119663, Krueppel-like factor 14, Kruppel-like factor 14, MGC119662, MGC119664, MGC119665, Sp6 transcription factor, transcription factor Sp6

Gene Symbol

SP6

Additional SP6 Products

Product Documents for SP6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SP6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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