![Western Blot: SPARC Antibody [NBP2-27358]](https://resources.rndsystems.com/images/products/SPARC-Antibody-Western-Blot-NBP2-27358-img0001.jpg "Western Blot: SPARC Antibody [NBP2-27358]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (97%), Orangutan (97%), Porcine (92%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
A portion of amino acids 25-85 of human SPARC was used as the immunogen for the antibody. (aa46-63).
Reactivity Notes
The amino acid sequence used as immunogen is 86% homologous in rabbit.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SPARC Antibody
Western Blot: SPARC Antibody [NBP2-27358]
Western Blot: SPARC Antibody [NBP2-27358] - Analysis of human A375 lysate probed with SPARC antibody. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.![Immunohistochemistry-Paraffin: SPARC Antibody [NBP2-27358]](https://resources.rndsystems.com/images/products/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP2-27358-img0002.jpg "Immunohistochemistry-Paraffin: SPARC Antibody [NBP2-27358]")
Immunohistochemistry-Paraffin: SPARC Antibody [NBP2-27358]
Immunohistochemistry-Paraffin: SPARC Antibody [NBP2-27358] - FFPE human placenta tissue stained with SPARC antibody, peroxidase-conjugate and DAB chromogen.Applications for SPARC Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
5ug/ml
Western Blot
2-5ug/ml~
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SPARC
Long Name
Secreted Protein Acidic and Rich in Cysteine
Alternate Names
BM-40, Osteonectin
Gene Symbol
SPARC
UniProt
Additional SPARC Products
Product Documents for SPARC Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SPARC Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
