Spectrin beta 3 Antibody (3B1NB) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-44311

Novus Biologicals
Loading...

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 3B1NB

Format

Azide and BSA Free
Loading...

Product Specifications

Immunogen

Partial recombinant human Spectrin beta 3 protein (between amino acids 1300-1500) [UniProt O15020]

Localization

Cytoplasm, Cytoskeleton, Cell junctions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for Spectrin beta 3 Antibody (3B1NB) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311]

Immunocytochemistry/ Immunofluorescence: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311]

Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Spectrin beta 3 (3B1NB) conjugated to Alexa Fluor 488 [NBP2-44310AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311]

Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311]

Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human prostate using 1:200 dilution of Spectrin beta 3 antibody (clone 3B1NB). The antibody generated a specific cytoplasmic staining with strong signal at cell-cell junctions in the glandular epithelium of the prostate section. The cells of fibromuscular stroma showed a diffused signal throughout the sections. Image using the standard format of this product.
Flow (Cell Surface): Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311]

Flow (Cell Surface): Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311]

Flow (Cell Surface): Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - Spectrin B3 antibody (clone 3B1NB) was used to stain HeLa cells (1 x 10^6 cells/ml) at a 1:500 dilution detected using Dylight-488 conjugated goat anti-rat IgG secondary antibody (blue peak). Shown with secondary control (orange peak).
Immunocytochemistry/ Immunofluorescence: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311]

Immunocytochemistry/ Immunofluorescence: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311]

Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - A431 cells were fixed for 10 minutes using -20C MeOH. The cells were incubated with anti-Spectrin beta 3 (3B1NB) [NBP2-44311-azide free] at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Applications for Spectrin beta 3 Antibody (3B1NB) - Azide and BSA Free

Application
Recommended Usage

Flow (Cell Surface)

1:500

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

1:200-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

3-4 ug/ml

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-III Spectrin

Spectrin beta 3 (SPTBN2) belongs to spectrins family of proteins which are essential to mechanical support/structural membrane integrity maintenance in erythrocytes, stabilizing cell-cell contacts, and localizing ion channels as well as cell adhesion molecules within specific subdomains of plasma membranes. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Spectrin beta 3 interacts with EAAT4, the glutamate transporter predominately expressed in Purkinje cells, and stabilizes it at the plasma membrane leading to glutamate clearance from the synaptic cleft, and resulting in both modulation of glutamatergic neurotransmission as well as prevention of glutamate-mediated neurotoxicity. Defective spectrin beta 3 has been established as causing factor in spinocerebellar ataxia type 5 (SCA5).

Long Name

Spectrin Beta, Non-Erythrocytic 2

Alternate Names

GTRAP41, SCA5, SCAR14, Spectrin beta 3, Spectrin beta III, SPTBN2

Gene Symbol

SPTBN2

Additional beta-III Spectrin Products

Product Documents for Spectrin beta 3 Antibody (3B1NB) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Spectrin beta 3 Antibody (3B1NB) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Customer Reviews for Spectrin beta 3 Antibody (3B1NB) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Spectrin beta 3 Antibody (3B1NB) - Azide and BSA Free and earn rewards!

Have you used Spectrin beta 3 Antibody (3B1NB) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...