SPG20 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-54942

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: KEEAKNYYKQGIGHLLRGISISSKESEHTGPGWESARQMQQKMKETLQNVRTRLEILEKGLATSLQNDLQEVPKLYPEFPPKDMCEKLPEPQSFSSAPQ

Reactivity Notes

Mouse 84%, Rat 81%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPG20 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SPG20 Antibody [NBP2-54942]

Immunocytochemistry/ Immunofluorescence: SPG20 Antibody [NBP2-54942]

Immunocytochemistry/Immunofluorescence: SPG20 Antibody [NBP2-54942] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: SPG20 Antibody [NBP2-54942]

Immunohistochemistry-Paraffin: SPG20 Antibody [NBP2-54942]

Immunohistochemistry-Paraffin: SPG20 Antibody [NBP2-54942] - Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells.

Applications for SPG20 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPG20

The SPG20 gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with

Alternate Names

KIAA0610SPARTIN, spastic paraplegia 20 (Troyer syndrome), Spastic paraplegia 20 protein, TAHCCP1spartin, Trans-activated by hepatitis C virus core protein 1

Gene Symbol

SPART

Additional SPG20 Products

Product Documents for SPG20 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SPG20 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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