SPG7 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93364

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SPG7 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human SPG7 (NP_003110.1). MAVLLLLLRALRRGPGPGPRPLWGPGPAWSPGFPARPGRGRPYMASRPPGDLAEAGGRALQSLQLRLLTPTFEGINGLLLKQHLVQNPVRLWQLLGGTFYFNTSRLKQKNKEKDKSKGKAPEEDEEERRRRERDDQMYRERLRTLLVIAVVMSLLNALSTSGGSISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIEAKDRIPVSYKRTGF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPG7 Antibody - BSA Free

SPG7 Antibody - BSA Free

Western Blot: SPG7 Antibody - BSA Free [NBP2-93364] -

Western Blot: SPG7 Antibody - BSA Free [NBP2-93364] - Western blot analysis of various lysates using SPG7 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates / proteins: 25 ug per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
SPG7 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SPG7 Antibody - BSA Free [NBP2-93364] -

Immunocytochemistry/ Immunofluorescence: SPG7 Antibody - BSA Free [NBP2-93364] - Immunofluorescence analysis of HepG2 cells using SPG7 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
SPG7 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SPG7 Antibody - BSA Free [NBP2-93364] -

Immunocytochemistry/ Immunofluorescence: SPG7 Antibody - BSA Free [NBP2-93364] - Immunofluorescence analysis of PC-12 cells using SPG7 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for SPG7 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SPG7

SPG7 - spastic paraplegia 7, paraplegin (pure and complicated autosomal recessive)

Alternate Names

CARMGC126331, cell matrix adhesion regulator, CMARMGC126332, EC 3.4.24, EC 3.4.24.-, FLJ37308, paraplegin, PGNcell adhesion regulator, spastic paraplegia 7 (pure and complicated autosomal recessive), Spastic paraplegia 7 protein, SPG5C

Gene Symbol

SPG7

Additional SPG7 Products

Product Documents for SPG7 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SPG7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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