Sphingomyelin Synthase 2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-30889

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Sphingomyelin Synthase 2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide derived from the human Sphingomyelin Synthase 2 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sphingomyelin Synthase 2 Antibody - Azide and BSA Free

Sphingomyelin Synthase 2 Antibody

Western Blot: Sphingomyelin Synthase 2 Antibody [NBP3-30889] -

Western Blot: Sphingomyelin Synthase 2 Antibody [NBP3-30889] - Fig 1. Western blot on human brain lysate (10 ug/lane) using Novus Biologicals' NBP3-30889 antibody to Sphingomyelin Synthase 2 (0.5 ug/ml). Lane A] 1 ug/ml antibody alone, lane B] antibody plus 3 ug blocking peptide. Blot was developed with Pierce’s Super System West Femto - 1 minute exposure. Fig 2. Human kidney section stained with Novus Biologicals' anti-SMS-2 antibody (Cat NBP3-30889) at 2 ug/ml. Anti-rabbit Ig H+L HRP conjugate (5 ug/ml) without antigen retrieval.

Applications for Sphingomyelin Synthase 2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

: 1:400

Western Blot

: 1:400
Application Notes
.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Phosphate buffered saline, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sphingomyelin Synthase 2

Sphingomyelin (SM) is a major component of plasma membranes. It is preferentially concentrated in the outer leaflet and has a role in the formation of lipid rafts. SM synthases (EC 2.7.8.27), such as SGMS2, produce SM in the lumen of the Golgi and on the cell surface through the transfer of phosphocholine from phosphatidylcholine onto ceramide, yielding diacylglycerol as a side product (Huitema et al., 2004 [PubMed 14685263]).[supplied by OMIM]

Alternate Names

EC 2.7.8.27, phosphatidylcholine:ceramide cholinephosphotransferase 2, SM synthase, sphingomyelin synthase 2SMS2MGC26963

Gene Symbol

SGMS2

Additional Sphingomyelin Synthase 2 Products

Product Documents for Sphingomyelin Synthase 2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Sphingomyelin Synthase 2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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