Sphingosine Kinase 1/SPHK1 Antibody (JA31-14)

Novus Biologicals | Catalog # NBP2-67164

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JA31-14 expressed in HEK293
View all Sphingosine Kinase 1/SPHK1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Sphingosine Kinase 1/SPHK1 aa 25-74 / 384. (SwissProt: Q9NYA1 Human)

Localization

Cytoplasm. Nucleus. Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sphingosine Kinase 1/SPHK1 Antibody (JA31-14)

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164]

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164]

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Western blot analysis of Sphingosine Kinase 1/SPHK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate
Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164]

Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164]

Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of paraffin-embedded human kidney tissue using anti-SPHK1 antibody. Counter stained with hematoxylin.
Flow Cytometry: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164]

Flow Cytometry: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164]

Flow Cytometry: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of Raji cells with SPHK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164]

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164]

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of SPHK1 on C2C12 lysates using anti-SPHK1 antibody at 1/1,000 dilution.
Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164]

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164]

Western blot analysis on different lysates with NBP2-67164 at 1/5,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: RAW264.7 cell lysate (20 µg/Lane) Lane 5: Mouse lung tissue lysate (30 µg/Lane) Lane 6: Mouse skeletal muscle tissue lysate (negative) (30 µg/Lane) Predicted band size: 43 kDa Observed band size: 43-51 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67164 at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for Sphingosine Kinase 1/SPHK1 Antibody (JA31-14)

Application
Recommended Usage

Flow Cytometry

1:50

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sphingosine Kinase 1/SPHK1

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Alternate Names

SK1, SPK1

Gene Symbol

SPHK1

Additional Sphingosine Kinase 1/SPHK1 Products

Product Documents for Sphingosine Kinase 1/SPHK1 Antibody (JA31-14)

Certificate of Analysis

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Product Specific Notices for Sphingosine Kinase 1/SPHK1 Antibody (JA31-14)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways
VEGF - VEGF R2 Signaling Pathway Thumbnail