Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-22974

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Sphingosine Kinase 1/SPHK1 Primary Antibodies »

Product Specifications

Immunogen

sphingosine kinase 1

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28175299).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974]

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974]

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974] - SPHK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- SPHK1 antibody NBP1-22974 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1 antibody NBP1-22973, which recognizes an upstream epitope

Applications for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

5 -15 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
This antibody is useful for western blot and immunoprecipitation. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:28175299).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Sphingosine Kinase 1/SPHK1

Sphingosine kinase 1 (SPHK1) catalyzes the phosphorylation of sphingosine to yield sphingosine-1-phosphate (SPP). SPP functions as a lipid signaling molecule that mediates both intra- and extra-cellular responses. SPHK1 activity may play a role in several cellular processes such as apoptisis, proliferation, inflammation, and migration.

Alternate Names

SK1, SPK1

Entrez Gene IDs

8877 (Human)

Gene Symbol

SPHK1

UniProt

Q2M3C7 (Human)

Additional Sphingosine Kinase 1/SPHK1 Products

Product Documents for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

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Protocols

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail