Spt6 Antibody

Novus Biologicals | Catalog # NB100-2582

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Chicken

Cited:

Human, Mouse, Avian - Chicken

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Knockdown Validated

Cited:

Western Blot, Immunoprecipitation, Chemotaxis, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Spt6 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 425 and 475 of human Suppressor of Ty 6 Homolog using the numbering given in entry NP_003161.2 (GeneID 6830).

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 23008333).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Spt6 Antibody

Western Blot: Spt6 Antibody [NB100-2582]

Western Blot: Spt6 Antibody [NB100-2582]

Western Blot: Spt6 Antibody [NB100-2582] - siRNA knockdown to confirm the specificity of Spt6 antibody (NB100-2582) in C2C12 myoblasts, WB: 1:2000 dilution. 20 ug whole cell lysate. WB image submitted by a verified customer review.
Spt6 Antibody

Spt6 Antibody [NB100-2582] -

Whole cell lysate (50 µg) from HEK293T, K-562,HeLa, TCMK-1, and NIH 3T3 cells prepared using NETNlysis buffer. Antibody: Affinity purified rabbit anti-SUPT6Hantibody used for WB at0.04 µg/ml. Detection: Chemiluminescence with anexposure time of 3 minutes.
Spt6 Antibody

Western Blot: Spt6 Antibody [NB100-2582] -

Western Blot: Spt6 Antibody [NB100-2582] - SPT6 Depletion Induces lncRNA Transcription(A) YWHAZ locus showing pre-mRNA (– strand) & PROMPT (+ strand). SPT6 depletion induced lncRNA as shown by ChRNA-seq & mNET-seq analyses. The profile of mNuc-seq/H3K36me3 is shown below.(B) Meta-analysis of strand-specific ChrRNA-seq signals from −3 kb of TSS to +3 kb of transcription end site (TES) for divergent (pre-mRNA-PROMPT) genes.(C) Boxplots of PROMPT mNET-seq/total CTD & T4P signals.(D) Enhancer located 90 kb downstream of NR4A1 gene with neighboring gene ATG101 showing SPT6 depletion-induced eRNA by ChRNA-seq & mNET-seq. mNuc-seq/H3K4me1 & H3K4me3 signals indicate active enhancer & promoter, respectively.(E) Meta-analysis of eRNA from ChrRNA-seq & mNET-seq (Total) −3 kb to +3 kb from TSS (enhancers with highest eRNA levels selected).(F) Boxplots of eRNA mNET-seq/total CTD & T4P signals at TSS (–/+2 kb).(G & H) Western blot (G) & quantitative RT-PCR (H) of chromatin-bound RNA of parental & SETD2 CRISPR KO U2OS cells with indicated siRNA transfection for 48 hr. Data are represented as mean ± SEM.See also Figure S3. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30449723), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Spt6 Antibody

Western Blot: Spt6 Antibody [NB100-2582] -

Western Blot: Spt6 Antibody [NB100-2582] - SPT6 Depletion Causes H3K36me3 Redistribution(A) Western blots with indicated antibodies 60 hr post-siSPT6 transfection (versus siLuc nonspecific control). EXOSC3 & H3 profiles shown as loading controls.(B) Meta-analysis of reads density (FPKM) for ratio of mNuc-seq/H3K36me3 with H3 at TSS of divergent PROMPT-pre-mRNA & enhancer RNA (eRNA) following SPT6 depletion. All subsequent transcription images employing siSPT6 versus siLuc are shown in red & blue, respectively.(C) Scatterplots of H3K36me3/H3 on pre-mRNA (0 to +3 kb), PROMPT (−3 kb to 0), & eRNA (−3 kb to +3 kb) regions in siLuc versus siSPT6. The percentage of upregulated regions by SPT6 depletion are indicated.(D) Boxplots of mNuc-seq/H3K36me3 ratio across pre-mRNA gene bodies, PROMPTs (3 kb from TSS), & eRNA (2 kb from center) & across lincRNA gene bodies.(E) Model of redistributed H3K36me3 marks caused by SPT6 depletion.See also Figure S2. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30449723), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Spt6 Antibody

Spt6 Antibody [NB100-2582] -

Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HEK293T cellsprepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-SUPT6H antibody) used for IP at 6 µg per reaction. SUPT6Hwas also immunoprecipitated by a previous lot of thisantibody. For blottingimmunoprecipitated SUPT6H, was used at 0.04µg/ml. Detection: Chemiluminescence with an exposuretime of 75 seconds.
Spt6 Antibody

Spt6 Antibody [NB100-2582] -

Section of human breast carcinoma.Antibody: Affinity purified rabbit anti- SUPT6H used at a dilution of 1:200 (1µg/ml).Detection: DAB
Spt6 Antibody

Spt6 Antibody [NB100-2582] -

Section of mouse plasmacytoma. Antibody:Affinity purified rabbit anti- SUPT6H used at a dilution of 1:200 (1µg/ml). Detection: DAB

Applications for Spt6 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Reported in scientific literature (PMID:23503590).

Immunohistochemistry

1:100 to 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Immunoprecipitation

2 - 10 µg/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 2 reviews rated 5 using NB100-2582 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Spt6

Spt6 is a histone H3-H4 chaperone protein that mediates chromatin reassembly behind RNA polymerase II. Spt6 binds both histone H3 and H4 and deposits them on to DNA, which leads to transcriptional repression of target genes.

Alternate Names

emb-5, hSPT6, KIAA0162MGC87943, SPT6HSPT6, suppressor of Ty (S.cerevisiae) 6 homolog, suppressor of Ty 6 homolog (S. cerevisiae), Tat-cotransactivator 2 protein, Tat-CT2 protein, transcription elongation factor SPT6

Entrez Gene IDs

6830 (Human)

Gene Symbol

SUPT6H

UniProt

Q7KZ85 (Human)

Additional Spt6 Products

Product Documents for Spt6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Spt6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Spt6 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Spt6 Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: breast cancer cell line, Sample Amount: 40
    Species: Human
    Verified Customer | Posted 03/06/2021
    MDA-MB-231 or MCF-7 cell lysates, 40ug, separated on 10% SDS-PAGE, SPT6 primary antibody (NB100-2582), 1:1000, overnight.
    Spt6 Antibody NB100-2582
  • Spt6 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse myoblasts (C2C12 cell lysate) and 20 ug whole cell lysate
    Species: Mouse
    Verified Customer | Posted 04/17/2018
    siRNA knockdown to confirm the specificity of Spt6 antibody (NB100-2582) in C2C12 myoblasts, WB: 1:2000 dilution. 20ug whole cell lysate.
    Spt6 Antibody NB100-2582

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