SRD5A2 Antibody (8A4H5)

Novus Biologicals | Catalog # NBP3-15900

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8A4H5 expressed in HEK293
View all SRD5A2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SRD5A2 (P31213). MQVQCQQSPVLAGSATLVALGALALYVAKPSGYGKHTESLKPAATRLPARAAWFLQELPSFAVPAGILARQPLSLFGPPGTVLLGLFCVHYFHRTFVYSL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SRD5A2 Antibody (8A4H5)

Western Blot: SRD5A2 Antibody (8A4H5) [NBP3-15900]

Western Blot: SRD5A2 Antibody (8A4H5) [NBP3-15900]

Western Blot: SRD5A2 Antibody (8A4H5) [NBP3-15900] - Analysis of extracts of various cell lines, using SRD5A2 Rabbit mAb (NBP3-15900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
SRD5A2 Antibody (8A4H5)

Immunohistochemistry-Paraffin: SRD5A2 Antibody (8A4H5) [NBP3-15900] -

Immunohistochemistry-Paraffin: SRD5A2 Antibody (8A4H5) [NBP3-15900] - Analysis of paraffin-embedded mouse testis using SRD5A2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
SRD5A2 Antibody (8A4H5)

Immunohistochemistry-Paraffin: SRD5A2 Antibody (8A4H5) [NBP3-15900] -

Immunohistochemistry-Paraffin: SRD5A2 Antibody (8A4H5) [NBP3-15900] - Analysis of paraffin-embedded rat testis using SRD5A2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for SRD5A2 Antibody (8A4H5)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:100 - 1:400

Immunohistochemistry-Paraffin

1:500

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SRD5A2

SRD5A2 encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

Long Name

3-oxo-5-alpha-steroid 4-dehydrogenase 2

Alternate Names

5 alpha-SR2, S5AR 2, SR type 2

Gene Symbol

SRD5A2

Additional SRD5A2 Products

Product Documents for SRD5A2 Antibody (8A4H5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SRD5A2 Antibody (8A4H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SRD5A2 Antibody (8A4H5)

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Protocols

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FAQs

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