SREBP1 Antibody (2A4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80959

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat, Canine, Chicken, Golden Syrian Hamster, Hamster, Monkey

Applications

Knockout Validated, Immunohistochemistry, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A4

Format

Azide and BSA Free
View all SREBP1 Primary Antibodies »

Product Specifications

Immunogen

6 His-tag fusion protein of human SREBP1 corresponding to amino acids 301-407. [UniProt# P36956]

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 23720350). Hamster reactivity reported in scientific literature (PMID: 24393244). Chicken reactivity reported in multiple pieces scientific literature. Monkey reactivity reported in scientific literature (PMID: 26437365).

Localization

Cytoplasmic and nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for SREBP1 Antibody (2A4) - Azide and BSA Free

Western Blot: SREBP1 Antibody (2A4)Azide and BSA Free [NBP2-80959]

Western Blot: SREBP1 Antibody (2A4)Azide and BSA Free [NBP2-80959]

Western Blot: SREBP1 Antibody (2A4) - Azide and BSA Free [NBP2-80959] - Hepatic SREBP1c and SREBP2 protein abundance in C57BL/6 mice assigned to a high-fat (HF) or a high-fat/exercise (HF + EX) group for 8 weeks. Cytosolic and nuclear SREBP1c abundance. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/jl/2013/908048/), licensed under a CC-BY license. Image from the standard format of this antibody.
Western Blot: SREBP1 Antibody (2A4)Azide and BSA Free [NBP2-80959]

Western Blot: SREBP1 Antibody (2A4)Azide and BSA Free [NBP2-80959]

Western Blot: SREBP1 Antibody (2A4) - Azide and BSA Free [NBP2-80959] - Analysis of whole cell lysate from HeLa cells showing a single specific band for the expression of SREBP1 precursor protein (~120 kDa). Image from the standard format of this antibody.
Simple Western: SREBP1 Antibody (2A4)Azide and BSA Free [NBP2-80959]

Simple Western: SREBP1 Antibody (2A4)Azide and BSA Free [NBP2-80959]

Simple Western: SREBP1 Antibody (2A4) - Azide and BSA Free [NBP2-80959] - Lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.
Knockout Validated: SREBP1 Antibody (2A4) - Azide and BSA Free [NBP2-80959]

Western Blot: SREBP1 Antibody (2A4) - Azide and BSA Free [NBP2-80959]

Western Blot: SREBP1 Antibody (2A4) - Azide and BSA Free [NBP2-80959] - KO and OE of SREBP1 influenced lipid metabolism in Bel-7402 cells. The protein level of lipid-associated genes in SREBP1-KO, SREBP1-OE, and WT Bel-7402 cells. Data were expressed as mean -/+ standard error of the mean. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. WT group, respectively. Image from the standard format of this antibody.

Applications for SREBP1 Antibody (2A4) - Azide and BSA Free

Application
Recommended Usage

Simple Western

1:12.5

Western Blot

1-2ug/ml
Application Notes
This SREBP1 (clone 2A4) antibody is useful for WB where a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:12.5. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SREBP1

SREBP1 (sterol regulatory element binding protein 1, also called SREBF1 or BHLHD1) is a bHLH-LZ (basic helix-loop-helix-leucine zipper) transcription factor that acts as a key lipogenic transcription factor controlled by glucose/insulin and preferentially regulates lipogenesis via activating genes involved in fatty acid as well as triglyceride synthesis. It is a transcriptional activator required for lipid homeostasis and regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. SREBP1 has a dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (sterol regulatory element 1; 5'-ATCACCCCAC-3'). It exists as multi-pass membrane protein in the membranes of ER, Golgi apparatus and cytoplasmic vesicle /COPII-coated vesicles and moves from ER to Golgi in the absence of sterols. It forms a tight complex with SCAP in ER membranes and interacts with LMNA. AMPK phosphorylate SREBP1 leading to suppression of protein processing and nuclear translocation, and target gene expression repression. Moreover, SIK1 mediated phosphorylation at Ser-402 site represses its activity through inhibition of DNA-binding.

Long Name

Sterol regulatory element-binding protein 1

Alternate Names

bHLHd1, SREBF1, SREBP-1

Gene Symbol

SREBF1

Additional SREBP1 Products

Product Documents for SREBP1 Antibody (2A4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SREBP1 Antibody (2A4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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