SREBP1 Antibody

Novus Biologicals | Catalog # NB100-74542

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SREBP1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues M(32) L Q L I N N Q D S D F P G L F(47) of mouse SREBP 1.

Localization

Cell Membrane, Endoplasmic reticulum, Golgi Apparatus and Nuclear. Multi-pass membrane protein. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SREBP1 Antibody

Western Blot: SREBP1 Antibody [NB100-74542]

Western Blot: SREBP1 Antibody [NB100-74542]

Western Blot: SREBP1 Antibody [NB100-74542] - Analysis of 25 ug of mouse liver (lane 1), rat liver (lane 2) and HepG2 (lane 3) cell lysates onto an SDS polyacrylamide gel.
Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-74542]

Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-74542]

Immunocytochemistry/Immunofluorescence: SREBP1 Antibody [NB100-74542] - Analysis of SREBP1 (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-74542]

Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-74542]

Immunocytochemistry/Immunofluorescence: SREBP1 Antibody [NB100-74542] - Analysis of SREBP1 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-74542]

Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-74542]

Immunocytochemistry/Immunofluorescence: SREBP1 Antibody [NB100-74542] - Analysis of SREBP1 (green) showing staining in the cytoplasm of HepG2 cells (right) compared to a negative control without primary antibody (left).

Applications for SREBP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500 - 1:5000
Application Notes
WB: Detects an approx. 68 and 120 kDa protein representing SREBP 1 in mouse and rat liver samples as well as rat kidney samples. A predominent band at ~68 kDa (active cleaved site) is seen and a band at ~120 kDa (inactive precursor) may not be seen or it may be diminished.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SREBP1

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

Long Name

Sterol regulatory element-binding protein 1

Alternate Names

bHLHd1, SREBF1, SREBP-1

Entrez Gene IDs

20787 (Mouse); 78968 (Rat)

Gene Symbol

SREBF1

UniProt

Q9WTN3 (Mouse)

Additional SREBP1 Products

Product Documents for SREBP1 Antibody

Certificate of Analysis

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Product Specific Notices for SREBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SREBP1 Antibody

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Protocols

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