SRp55 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-04142
![Western Blot: SRp55 Antibody [NBP2-04142]](https://resources.rndsystems.com/images/products/SRp55-Antibody-Western-Blot-NBP2-04142-img0013.jpg "Western Blot: SRp55 Antibody [NBP2-04142]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to this antibody using the numbering given in entry NP_006266.2 (GeneID 6431).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SRp55 Antibody - BSA Free
Western Blot: SRp55 Antibody [NBP2-04142]
Western Blot: SRp55 Antibody [NBP2-04142] - CEM-T4 and HeLa whole cell lysates. Image from verified customer review.![Immunocytochemistry/ Immunofluorescence: SRp55 Antibody [NBP2-04142]](https://resources.rndsystems.com/images/products/SRp55-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-04142-img0014.jpg "Immunocytochemistry/ Immunofluorescence: SRp55 Antibody [NBP2-04142]")
Immunocytochemistry/ Immunofluorescence: SRp55 Antibody [NBP2-04142]
Immunocytochemistry/Immunofluorescence: SRp55 Antibody [NBP2-04142] - Staining in human fibroblasts. 1X PBS/5% normal serum/0.3% Triton™ X-100. Diluted 1:200 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation. 2 hours (25C) secondary (1.5:1000 dilution). Image submitted by a verified customer review.![Immunohistochemistry-Paraffin: SRp55 Antibody [NBP2-04142]](https://resources.rndsystems.com/images/products/SRp55-Antibody-Immunohistochemistry-NBP2-04142-img0012.jpg "Immunohistochemistry-Paraffin: SRp55 Antibody [NBP2-04142]")
Immunohistochemistry-Paraffin: SRp55 Antibody [NBP2-04142]
Immunohistochemistry-Paraffin: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).![Western Blot: SRp55 Antibody [NBP2-04142]](https://resources.rndsystems.com/images/products/SRp55-Antibody-Western-Blot-NBP2-04142-img0010.jpg "Western Blot: SRp55 Antibody [NBP2-04142]")
Western Blot: SRp55 Antibody [NBP2-04142]
Western Blot: SRp55 Antibody [NBP2-04142] - analysis of SRp55 in human lymphocytes using anti-SRp55 antibody. Each lane loaded with 14ug of whole cell lysate with 3 different human B-cell lines. Image from verified customer review.![Immunohistochemistry-Paraffin: SRp55 Antibody [NBP2-04142]](https://resources.rndsystems.com/images/products/SRp55-Antibody-Immunohistochemistry-NBP2-04142-img0011.jpg "Immunohistochemistry-Paraffin: SRp55 Antibody [NBP2-04142]")
Immunohistochemistry-Paraffin: SRp55 Antibody [NBP2-04142]
Immunohistochemistry-Paraffin: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).![Immunoprecipitation: SRp55 Antibody [NBP2-04142]](https://resources.rndsystems.com/images/products/SRp55-Antibody-Immunoprecipitation-NBP2-04142-img0002.jpg "Immunoprecipitation: SRp55 Antibody [NBP2-04142]")
Immunoprecipitation: SRp55 Antibody [NBP2-04142]
Immunoprecipitation: SRp55 Antibody [NBP2-04142] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells.Antibodies: Affinity purified rabbit anti-SRp55 antibody NBP2-04142 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRp55 was also immunoprecipitated by rabbit anti-SRp55 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for SRp55 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. SRp55 antibody validated for ICC/IF from a verified customer review.
Reviewed Applications
Read 3 reviews rated 4.3 using NBP2-04142 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SRp55
Alternate Names
B52, FLJ08061, pre-mRNA splicing factor SRP55, Pre-mRNA-splicing factor SRP55, serine/arginine-rich splicing factor 6, SFRS6, Splicing factor, arginine/serine-rich 6MGC5045, splicing factor, arginine/serine-rich, 55 kDa, SR splicing factor 6, SRP55arginine/serine-rich splicing factor 6
Gene Symbol
SRSF6
UniProt
Additional SRp55 Products
Product Documents for SRp55 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SRp55 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SRp55 Antibody - BSA Free
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Customer Reviews for SRp55 Antibody - BSA Free (3)
4.3 out of 5
3 Customer Ratings
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Application: Western BlotSample Tested: HeLa cell lysateSpecies: HumanVerified Customer | Posted 06/09/2018CEM-T4 and HeLa whole cell lysates probed for SRSF6 (SRp55) antibody
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Application: ImmunocytochemistrySample Tested: fibroblastsSpecies: HumanVerified Customer | Posted 11/18/20171X PBS/5% normal serum/0.3% Triton™ X-100 Diluted 1:200 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation. 2 hours (25C) secondary (1.5:1000 dilution) SRp55 in fibroblasts1X PBS/5% normal serum/0.3% Triton™ X-100 Diluted 1:200 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation. 2 hours (25C) secondary (1.5:1000 dilution)
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Application: Western BlotSample Tested: Human lymphocytesSpecies: HumanVerified Customer | Posted 01/21/2016SRp55 in Human Lymphocytes
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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