SSH1 [p Ser978] Antibody

Name: SSH1 [p Ser978] Antibody
Brand: Novus Biologicals
SKU: NBP3-23411
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23411

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all SSH1 Primary Antibodies »

Product Specifications

Immunogen

SSH1 (Ser-978) antibody was generated from a phospho-peptide that included amino acids surrounding serine 978 in human SSH1. This sequence has high homology to the conserved site in rat, mouse, chicken, and zebrafish SSH1L. The sequence also has high homology to the conserved site in SSH2L (Ser-1259). [UniProt# Q8WYL5]

Modification

p Ser978

Specificity

The antibody detects a 150 kDa full length recombinant human SSH1 phosphoprotein and this reactivity is eliminated with lambda phosphatase treatment. The antibody also detects SSH1 on immunoblots of rat PC12 and mouse C2C12 cells that were stimulated with calyculin A.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for SSH1 [p Ser978] Antibody

Western Blot: SSH1 [p Ser978] Antibody [NBP3-23411] -

Western Blot: SSH1 [p Ser978] Antibody [NBP3-23411] - Western blot of human recombinant SSH1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1 (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1 (Ser-978) (lanes 3, 4, 7, & 8).

Immunocytochemistry/Immunofluorescence: SSH1 [p Ser978] Antibody [NBP3-23411] -

Immunocytochemistry/Immunofluorescence: SSH1 [p Ser978] Antibody [NBP3-23411] - Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-SSH1 (C-term.) and anti-SSH1 (Ser-978) antibodies, then detected using appropriate secondary antibody conjugated to Cy3 (Right panel). Phase image of corresponding PC12 cells (Left panel).

Applications for SSH1 [p Ser978] Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: SSH1

SSH1, also known as slingshot, is a phosphatase that functions to activate the actin severing protein, ADF/cofilin, and influence actin dynamics. There are three members of the slingshot family, SSH1, SSH2, and SSH3. SSH1 has been shown to function downstream of alpha6beta4 integrin/Rac1 signaling in conjunction with 14-3-3 association.

Alternate Names

EC 3.1.3.16, EC 3.1.3.48, FLJ21928, hSSH-1L, KIAA1298FLJ38102, protein phosphatase Slingshot homolog 1, slingshot 1, slingshot homolog 1 (Drosophila), SSH1L, SSH-1L, SSH-like protein 1

Gene Symbol

SSH1

Additional SSH1 Products

Product Documents for SSH1 [p Ser978] Antibody

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Certificate of Analysis

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Product Specific Notices for SSH1 [p Ser978] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SSH1 [p Ser978] Antibody

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