ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15817

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Chimpanzee (99%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ST6 Gal Sialyltransferase 1/ST6GAL1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD75. The exact sequence is proprietary.

Reactivity Notes

Bovine (88%), Mouse (87%), Rat (87%), Sheep (89%).

Localization

Golgi apparatus; Golgi stack membrane; Single-pass type II membrane protein; Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody - BSA Free

Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817]

Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817]

Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] - Analysis of ST6GAL1 expression in transfected 293T cell line by ST6GAL1 polyclonal antibody. A: Non-transfected lysate. B: ST6GAL1 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817]

Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817]

Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] - Human hepatocellular carcinoma. CD75 antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817]

Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817]

Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] - Analysis of paraffin-embedded OVCA xenograft, using antibody at 1:500 dilution.
ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody

Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] -

Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CD75 antibody [N2C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ST6 Gal Sialyltransferase 1/ST6GAL1

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Long Name

ST6 beta-Galactoside alpha-2,6-Sialyltransferase 1

Alternate Names

Alpha 2,6-ST, B-cell antigen CD75, CD75, SIAT1, ST6N

Gene Symbol

ST6GAL1

UniProt

P15907

Additional ST6 Gal Sialyltransferase 1/ST6GAL1 Products

Product Documents for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Glycobiology-related Enzymes

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Protocols

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