STAM-1 Antibody (HL1635) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25518

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1635 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fragment of human STAM-1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STAM-1 Antibody (HL1635) - Azide and BSA Free

STAM-1 Antibody (HL1635) - Azide and BSA Free

Immunohistochemistry-Paraffin: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] -

Immunohistochemistry-Paraffin: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - STAM antibody [HL1635] detects STAM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. STAM stained by STAM antibody [HL1635] (NBP3-25518) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
STAM-1 Antibody (HL1635) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] -

Immunocytochemistry/Immunofluorescence: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - STAM antibody [HL1635] detects STAM protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: STAM stained by STAM antibody [HL1635] (NBP3-25518) diluted at 1:500. Blue: Fluoroshield with DAPI.
STAM-1 Antibody (HL1635) - Azide and BSA Free

Western Blot: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] -

Western Blot: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM antibody [HL1635] (NBP3-25518) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
STAM-1 Antibody (HL1635) - Azide and BSA Free

Western Blot: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] -

Western Blot: STAM-1 Antibody (HL1635) - Azide and BSA Free [NBP3-25518] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with STAM antibody [HL1635] (NBP3-25518) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for STAM-1 Antibody (HL1635) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:5000-1:100000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAM-1

STAM was identified by the rapid tyrosine-phosphorylation of its product in response to cytokine stimulation. The encoded protein contains a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). This protein associates with JAK3 and JAK2 kinases via its ITAM region, and is phosphorylated by the JAK kinases upon cytokine stimulation, which suggests the function of this protein is as an adaptor molecule involved in the downstream signaling of cytokine receptors. HGS/HRS (hepatocyte grwoth factor-regulated tyrosine kinase substrate) has been found to bind and counteract the function of this protein.

Long Name

Signal Transducing Adaptor Molecule 1

Alternate Names

HSE1 Homolog, STAM, STAM1

Gene Symbol

STAM

Additional STAM-1 Products

Product Documents for STAM-1 Antibody (HL1635) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for STAM-1 Antibody (HL1635) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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