STAM-1 Antibody (HL1637)

Novus Biologicals | Catalog # NBP3-48660

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1637
View all STAM-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human STAM-1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STAM-1 Antibody (HL1637)

STAM-1 Antibody (HL1637)

Immunocytochemistry/ Immunofluorescence: STAM-1 Antibody (HL1637) [NBP3-48660] -

STAM-1 antibody [HL1637] detects STAM-1 protein at endosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STAM-1 stained by STAM-1 antibody [HL1637] (NBP3-48660) diluted at 1:500.
Blue: Fluoroshield with DAPI.
STAM-1 Antibody (HL1637)

Western Blot: STAM-1 Antibody (HL1637) [NBP3-48660] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM-1 antibody [HL1637] (NBP3-48660) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
STAM-1 Antibody (HL1637)

Western Blot: STAM-1 Antibody (HL1637) [NBP3-48660] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (10 | 10 | 20 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM-1 antibody [HL1637] (NBP3-48660) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
STAM-1 Antibody (HL1637)

Western Blot: STAM-1 Antibody (HL1637) [NBP3-48660] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM-1 antibody [HL1637] (NBP3-48660) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for STAM-1 Antibody (HL1637)

Application
Recommended Usage

Western Blot

1:500-1:25000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAM-1

STAM was identified by the rapid tyrosine-phosphorylation of its product in response to cytokine stimulation. The encoded protein contains a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). This protein associates with JAK3 and JAK2 kinases via its ITAM region, and is phosphorylated by the JAK kinases upon cytokine stimulation, which suggests the function of this protein is as an adaptor molecule involved in the downstream signaling of cytokine receptors. HGS/HRS (hepatocyte grwoth factor-regulated tyrosine kinase substrate) has been found to bind and counteract the function of this protein.

Long Name

Signal Transducing Adaptor Molecule 1

Alternate Names

HSE1 Homolog, STAM, STAM1

Gene Symbol

STAM

Additional STAM-1 Products

Product Documents for STAM-1 Antibody (HL1637)

Certificate of Analysis

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Product Specific Notices for STAM-1 Antibody (HL1637)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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