Stargazin Antibody (S245-36) - BSA Free

Novus Biologicals | Catalog # NBP2-22411

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S245-36

Format

BSA Free
View all Stargazin Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 203-323 (Cytoplasmic C-terminus) of rat TARPGamma2

Localization

Membrane

Specificity

Detects approx 35-55kDa. Does not cross-react with TARPGamma3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Stargazin Antibody (S245-36) - BSA Free

Western Blot: Stargazin Antibody (S245-36) [NBP2-22411]

Western Blot: Stargazin Antibody (S245-36) [NBP2-22411]

Western Blot: Stargazin Antibody (S245-36) [NBP2-22411] - Western Blot analysis of Rat brain lysates showing detection of StargazinCalcium Channel protein using Mouse Anti-StargazinCalcium Channel Monoclonal Antibody, Clone S245-36 (NBP2-22411). Primary Antibody: Mouse Anti-StargazinCalcium Channel Monoclonal Antibody (NBP2-22411) at 1:100, 1:250, 1:500, and 1:1000.
Immunocytochemistry/ Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411]

Immunocytochemistry/ Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411]

Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (NBP2-22411). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (NBP2-22411) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411]

Immunocytochemistry/ Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411]

Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.
Immunocytochemistry/ Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411]

Immunocytochemistry/ Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411]

Immunocytochemistry/Immunofluorescence: Stargazin Antibody (S245-36) [NBP2-22411] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (NBP2-22411). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (NBP2-22411) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody. (D) Composite.

Applications for Stargazin Antibody (S245-36) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of TARP Gamma2/4/8 (Stargazin) Antibody was sufficient for detection of TARP Gamma2/4/8 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Stargazin

Stargazin is a member of the transmembrane AMPAR regulatory proteins (TARP) family and is involved in glutamate receptor trafficking. It has been recently demonstrated (Tomita et al., 2005; Priel et al., 2005) that the interaction between stargazin and AMPA receptors is critical for the correct localization of the receptors at the synapse. Phosphorylation of the stargazin protein at Thr 321 by Protein Kinase A regulates its interaction with PSD-95 and synaptic targeting of AMPA receptors (Choi et al., 2002). Stargazin mutant mice are utilized as a model for absence epilepsy.

Long Name

Voltage-dependent calcium channel gamma-2 subunit

Alternate Names

CACNG2, TARP gamma-2

Gene Symbol

CACNG2

UniProt

Q71RJ2 (Rat)

Additional Stargazin Products

Product Documents for Stargazin Antibody (S245-36) - BSA Free

Certificate of Analysis

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Product Specific Notices for Stargazin Antibody (S245-36) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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