STEAP1 Antibody (4F6-1F3) - Azide and BSA Free

Novus Biologicals | Catalog # H00026872-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4F6-1F3

Format

Azide and BSA Free
View all STEAP1 Primary Antibodies »

Product Specifications

Immunogen

STEAP1 (AAH11802, 1 a.a. ~ 339 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MESRKDITNQEELWKMKPRRNLEEDDYLHKDTGETSMLKRPVLLHLHQTAHADEFDCPSELQHTQELFPQWHLPIKIAAIIASLTFLYTLLREVIHPLATSHQQYFYKIPILVINKVLPMVSITLLALVYLPGVIAAIVQLHNGTKYKKFPHWLDKWMLTRKQFGLLSFFFAVLHAIYSLSYPMRRSYRYKLLNWAYQQVQQNKEDAWIEHDVWRMEIYVSLGIVGLAILALLAVTSIPSVSDSLTWREFHYIQSKLGIVSLLLGTIHALIFAWNKWIDIKQFVWYTPPTFMIAVFLPIVVLIFKSILFLPCLRKKILKIRHGWEDVTKINKTEICSQL

Specificity

STEAP1 - six transmembrane epithelial antigen of the prostate 1 (4F6-1F3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for STEAP1 Antibody (4F6-1F3) - Azide and BSA Free

Western Blot: STEAP1 Antibody (4F6-1F3) [H00026872-M01]

Western Blot: STEAP1 Antibody (4F6-1F3) [H00026872-M01]

Western Blot: STEAP1 Antibody (4F6-1F3) [H00026872-M01] - STEAP1 monoclonal antibody (M01), clone 4F6-1F3 Analysis of STEAP1 expression in A-431.
Immunocytochemistry/ Immunofluorescence: STEAP1 Antibody (4F6-1F3) [H00026872-M01]

Immunocytochemistry/ Immunofluorescence: STEAP1 Antibody (4F6-1F3) [H00026872-M01]

Immunocytochemistry/Immunofluorescence: STEAP1 Antibody (4F6-1F3) [H00026872-M01] - Analysis of monoclonal antibody to STEAP1 on A-431 cell. Antibody concentration 10 ug/ml.
Western Blot: STEAP1 Antibody (4F6-1F3) [H00026872-M01]

Western Blot: STEAP1 Antibody (4F6-1F3) [H00026872-M01]

Western Blot: STEAP1 Antibody (4F6-1F3) [H00026872-M01] - Analysis of STEAP1 expression in transfected 293T cell line by STEAP1 monoclonal antibody (M01), clone 4F6-1F3.Lane 1: STEAP1 transfected lysate (Predicted MW: 39.9 KDa).Lane 2: Non-transfected lysate.
ELISA: STEAP1 Antibody (4F6-1F3) [H00026872-M01]

ELISA: STEAP1 Antibody (4F6-1F3) [H00026872-M01]

ELISA: STEAP1 Antibody (4F6-1F3) [H00026872-M01] - Detection limit for recombinant GST tagged STEAP1 is approximately 0.3ng/ml as a capture antibody.

Applications for STEAP1 Antibody (4F6-1F3) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STEAP1

This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions.

Long Name

Six Transmembrane Epithelial Antigen of the Prostate 1

Alternate Names

PRSS24

Gene Symbol

STEAP1

OMIM

604415 (Human)

Additional STEAP1 Products

Product Documents for STEAP1 Antibody (4F6-1F3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for STEAP1 Antibody (4F6-1F3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Redox Enzymes

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for STEAP1 Antibody (4F6-1F3) - Azide and BSA Free

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  • Q: May any of your anti human-STEAP1 Abs be used for flow cytometry, with a secondary anti-mouse antibody? E.g. J2D2 or 4F6-1F3?

    A: Unfortunately, none of our STEAP1 antibodies have been validated in flow cytometry yet. This does not mean they will not work in FLOW, we just cannot guarantee its use in FLOW application because we have not tested them in this application in our lab yet. However, if you would like to try one of these antibodies in FLOW, you would be eligible for our Innovator's Reward program, whereby if you share your results with us, we will reward you with a voucher for 100% of the purchase price. Technically, STEAP1 (J2D2) NBP1-07094 and STEAP1 (4F6-1F3) H00026872-M01 are the two antibodies that have more chances of success because of proven compatibility with IF labeled secondary antibodies as they have already been validated in ICC-IF.

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