SUB1 Antibody

Novus Biologicals | Catalog # NB100-59774

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SUB1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human positive cofactor 4 using the numbering given in entry NP_006704.2 (GeneID 10923).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUB1 Antibody

Western Blot: SUB1 Antibody [NB100-59774]

Western Blot: SUB1 Antibody [NB100-59774]

Western Blot: SUB1 Antibody [NB100-59774] - Detection of Human PC4 on HeLa whole cell lysate using NB100-59774.
Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59774]

Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59774]

Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59774] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PC4 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for SUB1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30992017).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SUB1

PC4 is a multifunctional transcriptional coactivator that plays an important role in transcription, repair, and replication. PC4 interacts with general transcription factors, DNA repair factors, and mRNA processing factors to influence transcription initiation, coactivation, polyadenylation, transcription termination, and transcriptional repression. PC4 has been demonstrated to be a DNA binding protein and a bona fide component of chromatin with chromatin-compacting ability.

Alternate Names

activated RNA polymerase II transcription cofactor 4, activated RNA polymerase II transcriptional coactivator p15, MGC102747, p15, PC4p14P15, Positive cofactor 4, RPO2TC1, SUB1 homolog, SUB1 homolog (S. cerevisiae)

Entrez Gene IDs

10923 (Human)

Gene Symbol

SUB1

UniProt

NP_006704.2 (Human)

Additional SUB1 Products

Product Documents for SUB1 Antibody

Certificate of Analysis

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Product Specific Notices for SUB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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