Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80967

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B4

Format

Azide and BSA Free
View all Sulfatase-2/SULF2 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human Sulfatase 2 isoform b [Accession# NP_940998].

Localization

Endoplasmic reticulum

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free

Western Blot: Sulfatase-2/SULF2 Antibody (2B4)Azide and BSA Free [NBP2-80967]

Western Blot: Sulfatase-2/SULF2 Antibody (2B4)Azide and BSA Free [NBP2-80967]

Western Blot: Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free [NBP2-80967] - Analysis showing Sulfatase 2 expression in MCF7 conditioned media (CM). In CM, the 75 kDa band is dominant (Tang R, Rosen SD. J Biol Chem. 2009 Aug 7;284(32):21505-14). Image courtesy of Mark Singer at UCSF. Image from the standard format of this antibody.
Immunohistochemistry: Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free [NBP2-80967]

Immunohistochemistry: Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free [NBP2-80967]

Immunohistochemistry: Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free [NBP2-80967] - Immunohistochemical analysis of Sulfatase 2 in non-small-cell lung carcinoma. (left) lung squamous cell carcinoma stained with 2B4 antibody and (right) tumor-associated stromal cells stained with 2B4 antibody. Image courtesy of Mark Singer at UCSF. Image
Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free [NBP2-80967]

Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free [NBP2-80967]

Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free [NBP2-80967] - An intracellular stain was performed on HeLa cells with Sulfatase-2/SULF2 Antibody (2B4) NBP1-36727AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.
Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free [NBP2-80967]

Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free [NBP2-80967]

Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free [NBP2-80967] - Analysis using the FITC conjugate of NBP1-36727. Staining of Human lung cancer line with Sulfatase2-FITC. Image from verified customer review.

Applications for Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

reported by customer review on FITC-conjugated antibody.

Immunohistochemistry

1:250-1:500

Immunohistochemistry-Paraffin

1:250-1:500

Immunoprecipitation

1:10-1:100

Western Blot

1:1000
Application Notes
For WB a band can be seen at 50 kDa. Other bands may be seen at 135 kDa (unprocessed protein) and 37 kDa (fragment of 50 kDa subunit). For ELISA, use this antibody at 1-10 ug/ml for capture and 1-5 ug/ml for detection. It has been reported that NBP1-36727 picks up a 130 kDa band that is not Sulfatase 2 in human plasma. Because of this we do not recommend NBP1-36727 for capture of the human protein. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sulfatase-2/SULF2

Sulfatase 2 (SULF2) is a member of sulfatase family and catalyzes the removal of 6-O-sulfate groups from the heparan sulfate (HS) disaccharide units of heparan sulfate proteoglycans (HSPGs). HSPGs are present on the cell surface as well as in the extracellular matrix and act either as sequestration sites for growth factor/cytokine ligands or as coreceptors in multiple signal transduction pathways. SULF2 mediated desulfation of HSPGs is proposed to decrease the affinity of HSPGs for a variety of signaling ligands, releasing them from sequestration sites and making them available for triggering signaling stimuli. SULF2 is localized in endoplasmic reticulum, Golgi apparatus and also on the cell surface, and is expressed at highest levels in tissues such as ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta. In its post-translational modification, the conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. SULF2 enzyme regulates the activities of a number of signal transduction pathways which are up-regulated in many tissues during development and disease.

Alternate Names

SULF2, Sulfatase2

Gene Symbol

SULF2

Additional Sulfatase-2/SULF2 Products

Product Documents for Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Sulfatase-2/SULF2 Antibody (2B4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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