SUOX Antibody (OTI5A2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74411

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI5A2

Format

Azide and BSA Free
View all SUOX Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 320-545 of human SUOX(NP_000447) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

60.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SUOX Antibody (OTI5A2) - Azide and BSA Free

Western Blot: SUOX Antibody (OTI5A2)Azide and BSA Free [NBP2-74411]

Western Blot: SUOX Antibody (OTI5A2)Azide and BSA Free [NBP2-74411]

Western Blot: SUOX Antibody (OTI5A2) - Azide and BSA Free [NBP2-74411] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUOX.
Immunohistochemistry: SUOX Antibody (OTI5A2) - Azide and BSA Free [NBP2-74411]

Immunohistochemistry: SUOX Antibody (OTI5A2) - Azide and BSA Free [NBP2-74411]

Immunohistochemistry: SUOX Antibody (OTI5A2) - Azide and BSA Free [NBP2-74411] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUOX Antibody (OTI5A2) - Azide and BSA Free [NBP2-74411]

Immunohistochemistry: SUOX Antibody (OTI5A2) - Azide and BSA Free [NBP2-74411]

Immunohistochemistry: SUOX Antibody (OTI5A2) - Azide and BSA Free [NBP2-74411] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for SUOX Antibody (OTI5A2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SUOX

Sulfite oxidase is a homodimeric protein localized to the intermembrane space of mitochondria. Each subunit contains a heme domain and a molybdopterin-binding domain. The enzyme catalyzes the oxidation of sulfite to sulfate, the final reaction in the oxidative degradation of the sulfur amino acids cysteine and methionine. Sulfite oxidase deficiency results in neurological abnormalities which are often fatal at an early age. Alternative splicing results in multiple transcript variants encoding identical proteins.

Alternate Names

EC 1.8.3.1, sulfite oxidase, sulfite oxidase, mitochondrial

Gene Symbol

SUOX

Additional SUOX Products

Product Documents for SUOX Antibody (OTI5A2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SUOX Antibody (OTI5A2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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