Superoxide Dismutase Assay Kit

Analyzes Superoxide Dismutase (SOD) activity in cell extracts
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Citations (17)
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Superoxide Dismutase Assay Kit Summary

Analyzes Superoxide Dismutase (SOD) activity in cell extracts using cuvettes.

Key Benefits

• Suitable for mammalian cells.
• Each sample takes only 5 minutes.
• Contains SOD for 50 positive controls.
• Suitable for the assay of (Mn2+)-SOD, (Fe2+)-SOD, and (Cu/Zn)-SOD.

Why Use the Superoxide Dismutase Assay Kit?

In the Superoxide Dismutase Assay, ions generated from the conversion of xanthine to uric acid, and hydrogen peroxide by xanthine oxidase (XOD), convert NBT to NBT-diformazan. NBT-diformazan absorbs light at 550 nm. SODs reduce superoxide ion concentrations and thereby lower the rate of NBT-diformazan formation. The extent of reduction in the appearance of NBT-diformazan is a measure of SOD activity present in experimental samples. The assay is free of interference by other catalytic activities, and is ideal for assaying SOD in mammalian cell lysates. The kit contains the proper lysis buffer and the reagents needed for 100 experimental tests, 50 positive controls, and 50 negative controls. Unlike some other assay kits for SOD, this system is not greatly disturbed by trace metals. Each assay requires only about five minutes, and after a simple calculation, the percent inhibition of the formation of NBT-diformazan by SOD is converted to the relative activity of the sample.

Kit Contents

• Super Oxide Dismutase
• XOD Solution
• 25X SOD Reaction Buffer
• Xanthine Solution
• NBT Solution
• 20X Cell Lysis Solution

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Citations for Superoxide Dismutase Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Oxidative Stress, Lipid Peroxidation, and Loss of Hyaluronic Acid in the Human Vitreous Affected by Synchysis Scintillans
    Authors: L Bergandi, OA Skorokhod, R La Grotta, E Schwarzer, R Nuzzi
    J Ophthalmol, 2019;2019(0):7231015.  2019
  2. SOD2 Mediates Curcumin-Induced Protection against Oxygen-Glucose Deprivation/Reoxygenation Injury in HT22 Cells
    Authors: Y Wang, Y Zhang, L Yang, J Yuan, J Jia, S Yang
    Evid Based Complement Alternat Med, 2019;2019(0):2160642.  2019
  3. Calreticulin Ins5 and Del52 mutations impair unfolded protein and oxidative stress responses in K562 cells expressing CALR mutants
    Authors: S Salati, E Genovese, C Carretta, R Zini, N Bartalucci, Z Prudente, V Pennucci, S Ruberti, C Rossi, S Rontauroli, E Enzo, L Calabresi, M Balliu, C Mannarelli, E Bianchi, P Guglielmel, E Tagliafico, AM Vannucchi, R Manfredini
    Sci Rep, 2019;9(1):10558.  2019
  4. Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway
    Authors: XJ Chen, SM Ren, JZ Dong, CG Qiu, YW Chen, HL Tao
    Drug Des Devel Ther, 2019;13(0):647-655.  2019
  5. Anti-Hyperuricemic Effect of 2-Hydroxy-4-methoxy-benzophenone-5-sulfonic Acid in Hyperuricemic Mice through XOD
    Authors: T Yong, D Li, M Li, D Liang, X Diao, C Deng, S Chen, Y Xie, D Chen, D Zuo
    Molecules, 2018;23(10):.  2018
  6. Suppression of human arthritis synovial fibroblasts inflammation using dexamethasone-carbon nanotubes via increasing caveolin-dependent endocytosis and recovering mitochondrial membrane potential
    Authors: YK Lee, SW Kim, JY Park, WC Kang, YJ Kang, D Khang
    Int J Nanomedicine, 2017;12(0):5761-5779.  2017
  7. Pulmonary Biocompatibility Assessment of Inhaled Single-wall and Multiwall Carbon Nanotubes in BALB/c Mice.
    Authors: Ravichandran P, Baluchamy S, Gopikrishnan R, Biradar S, Ramesh V, Goornavar V, Thomas R, Wilson BL, Jeffers R, Hall JC, Ramesh GT
    J. Biol. Chem., 2011;286(34):29725-33.  2011
  8. Impaired response to oxidative stress in senescent cells may lead to accumulation of DNA damage in mesothelial cells from aged donors.
    Authors: Ksiazek K, Piatek K, Witowski J
    Biochem. Biophys. Res. Commun., 2008;373(2):335-9.  2008
  9. XIAP regulates intracellular ROS by enhancing antioxidant gene expression.
    Authors: Resch U, Schichl YM, Sattler S, de Martin R
    Biochem. Biophys. Res. Commun., 2008;375(1):156-61.  2008
  10. Inhibition of cadmium-induced oxidative injury in rat primary astrocytes by the addition of antioxidants and the reduction of intracellular calcium.
    Authors: Yang CS, Tzou BC, Liu YP, Tsai MJ, Shyue SK, Tzeng SF
    J. Cell. Biochem., 2008;103(3):825-34.  2008
  11. Antioxidant enzyme gene delivery to protect from HIV-1 gp120-induced neuronal apoptosis.
    Authors: Agrawal L, Louboutin JP, Reyes BA, Van Bockstaele EJ, Strayer DS
    Gene Ther., 2006;13(23):1645-56.  2006
  12. Oxidized SOD1 alters proteasome activities in vitro and in the cortex of SOD1 overexpressing mice.
    Authors: Le Pecheur M, Bourdon E, Paly E, Farout L, Friguet B, London J
    FEBS Lett., 2005;579(17):3613-8.  2005
  13. Rosiglitazone causes cardiotoxicity via peroxisome proliferator-activated receptor gamma-independent mitochondrial oxidative stress in mouse hearts.
    Authors: He H, Tao H, Xiong H, Duan S, McGowan F, Mortensen R, Balschi J
    Toxicol Sci, 0;138(2):468-81.  0
  14. CyclinB1/Cdk1 phosphorylates mitochondrial antioxidant MnSOD in cell adaptive response to radiation stress.
    Authors: Candas D, Fan M, Nantajit D, Vaughan A, Murley J, Woloschak G, Grdina D, Li J
    J Mol Cell Biol, 0;5(3):166-75.  0
  15. Oleanolic acid from antifilarial triterpene saponins of Dipterocarpus zeylanicus induces oxidative stress and apoptosis in filarial parasite Setaria digitata in vitro.
    Authors: Senathilake K, Karunanayake E, Samarakoon S, Tennekoon K, de Silva E, Adhikari A
    Exp Parasitol, 0;177(0):13-21.  0
  16. Protective activity of a novel resveratrol analogue, HS-1793, against DNA damage in 137Cs-irradiated CHO-K1 cells.
    Authors: Jeong M, Yang K, Jeong D, Lee C, Oh S, Jeong S, Lee K, Jo Y, Jo W
    J Radiat Res, 0;55(3):464-75.  0
  17. Genetic disruption of SOD1 gene causes glucose intolerance and impairs beta-cell function.
    Authors: Muscogiuri G, Salmon A, Aguayo-Mazzucato C, Li M, Balas B, Guardado-Mendoza R, Giaccari A, Reddick R, Reyna S, Weir G, Defronzo R, Van Remmen H, Musi N
    Diabetes, 0;62(12):4201-7.  0

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