Supplemental ELISA Development Products

Clear Microplates (Catalog # DY990)

A 25 pack of clear high-binding, flat-bottom polystyrene microplates (360 µL/well). Each 96-well microplate consists of 12 removable strips of 8 wells and a frame.

Black Microplates (Catalog # DY991)

A 25 pack of black high-binding, flat-bottom polystyrene microplates (360 µL/well) for use in chemiluminescent assays. Each 96-well microplate consists of 12 removable strips of 8 wells and a frame.

ELISA Plate Sealers (Catalog # DY992)

100 adhesive strips.

ELISA Plate-Coating Buffer (Catalog # DY006)

1 vial (250 mL) of sterile-filtered 1X PBS; for use as a plate-coating buffer in ELISA development.

Glo Substrate Reagent Pack (Catalog # DY993)

5 vials of Glo Reagent A (stabilized enhanced luminol, 4 mL/vial) and 5 vials of Glo Reagent B (stabilized hydrogen peroxide, 8 mL/vial). This reagent may be substituted for H2O2/tetramethylbenzidine as a substrate for horseradish peroxidase in ELISAs.

Stop Solution (Catalog # DY994)

15 vials (6 mL/vial) of 2N sulfuric acid. Add 50 - 100 µL/well to stop the action of horseradish peroxidase on the substrate, tetramethylbenzidine.

Reagent Diluent Concentrate 3 (Catalog # DY004)

10 vials (21 mL/vial) 25% Tween® 20 solution in PBS; for preparation of samples, standards, and detection reagents.

Reagent Diluent Concentrate 2 (Catalog # DY995)

5 vials (21 mL/vial) of a 10x concentrated BSA solution; for preparation of samples, standards, and detection reagents.

Reagent Diluent Concentrate 1 (Catalog # DY997)

1 vial (21 mL) of buffered protein base; for preparation of samples, standards, and detection reagents.

Reagent Additive 1 (Catalog # DY005)

1 vial (21 mL) of diluted, heat-inactivated normal goat serum; for preparation of Detection Antibody.

Streptavidin-HRP (Catalog # DY998)

5 vials (1 mL/vial) of streptavidin conjugated to horseradish peroxidase (HRP). For use in ELISAs, dilute to the working concentration specified on the vial label using 1% BSA/PBS or Reagent Diluent Concentrate 2 (Catalog # DY995).

Substrate Reagent Pack (Catalog # DY999)

8 vials of Color Reagent A (12.5 mL/vial stabilized hydrogen peroxide) and 8 vials of Color Reagent B (12.5 mL/vial stabilized tetramethylbenzidine). Reagents A and B are mixed 1:1 to form the DuoSet® IC Substrate Buffer (100 mL required per test well).

Wash Buffer Concentrate (Catalog # WA126)

5 vials (100 mL/vial) of 25X concentrated solution of buffered surfactant with preservatives. Dilute 20 mL of Wash Buffer Concentrate to a final volume of 500 mL in deionized water.

Sample Diluent Concentrate 1 (5X) (Catalog # DYC001)

5 vials (21 mL/vial) of a 5-fold concentrated solution. Sample Diluent Concentrate 1 (5X) is the base buffer for DuoSet® IC Diluents and Lysis Buffers. Please refer to product insert for detailed preparation information.

Sample Diluent Concentrate 1 (2X) (Catalog # DYC002)

1 bottle (250 mL) of a 2-fold concentrated solution. Sample Diluent Concentrate 1 (5X) is the base buffer for DuoSet® IC Diluents and Lysis Buffers. Please refer to product insert for detailed preparation information.

EvenCoat™ Goat Anti-mouse IgG Microplates (Catalog # CP001, CP002)

Pre-blocked 96-well polystyrene microplates coated with goat-derived antibody specific for the Fc region of mouse IgG. Available in packs of 5 microplates (Catalog # CP001) and 15 microplates (Catalog # CP002).

EvenCoat™ Streptavidin Microplates (Catalog # CP003, CP004)

Pre-blocked 96-well polystyrene microplates specific for biotin and biotin-conjugated compounds. Available in packs of 5 microplates (Catalog # CP003) and 15 microplates (Catalog # CP004). These plates may be used as a solid support for assays that utilize a biotinylated molecule or otherwise require the binding of biotin. Examples include immunoassays (biotinylated antibody or antigen), the measurement of DNA or RNA (biotinylated oligonucleotide), transcription factor or DNA binding proteins assays (biotinylated DNA), and kinase assays (biotinylated peptide).